IL-11Rα介导的分子探针在宫颈癌模型中的显像研究

发布时间：2018-02-04 15:36

  本文关键词： 白介素-受体 核素标记探针 核素成像 宫颈癌 小鼠　出处：《东南大学学报(医学版)》2017年06期 　论文类型：期刊论文

【摘要】：目的:探讨IL~(-1)1Rα介导的核素标记探针~(99)Tc~m-DTPA-c(CGRRAGGSC)在宫颈癌模型中的显像特性。方法:采用Western Blot法检测宫颈癌Hela细胞株IL~(-1)1Rα表达水平,免疫荧光进行HELA细胞体外结合实验;然后合成核素标记探针,对其标记率及稳定性进行检测,最后进行荷瘤裸鼠模型SPECT显像。结果:IL~(-1)1Rα在HELA细胞中呈明显高表达;合成探针即刻标记率达96.62%,放置于室温和人新鲜血清孵育12 h后放射化学纯度仍在90%;通过瘤体间质注射~(99)Tc~m-DTPA-c(CGRRAGGSC)见肿瘤局部呈明显的放射性浓聚且持续较长时间,而游离~(99)Tc~mO_4~-注射于瘤体内30 min即见肿瘤浓聚部位浅淡。结论:标记探针可由IL~(-1)1Rα介导对宫颈癌进行特异靶向性显像,有望为高表达IL~(-1)1Rα的恶性肿瘤提供早期、特异诊断及靶向治疗。
[Abstract]:Objective: to investigate the IL~(-1)1R 伪 -mediated radionuclide labeled probe TcmDTPA-cr rRAGSCC. Methods: Western Blot assay was used to detect the expression of IL~(-1)1R 伪 in cervical cancer Hela cell line. Immunofluorescence assay was performed on HELA cells in vitro. Then the radionuclide labeled probe was synthesized and its labeling rate and stability were tested. Finally, SPECT imaging of the nude mice bearing tumor was performed. Results the expression of 1 R 伪 in HELA cells was significantly higher than that in the control group. The immediate labeling rate of the synthetic probe was 96.62%, and the radiochemical purity was still 90 after being incubated at room temperature with human fresh serum for 12 hours. Through interstitial injection of TcmDTPA-cGSCC, it was found that the tumor area was radioactive and concentrated and lasted for a long time. However, 30 min after injection into the tumor body, the concentration of the tumor was observed. Conclusion: the labeled probe can be labeled by ILO-1). 1R 伪 mediated specific targeting imaging of cervical cancer. It is expected to provide early, specific diagnosis and targeted therapy for malignant tumors with high expression of IL~(-1)1R 伪.
【作者单位】： 东南大学医学院;东南大学附属中大医院放射科与核医学科;南京医科大学;南京医科大学附属无锡妇幼保健院妇科;
【基金】：国家自然科学基金资助项目(81372480)
【分类号】：R-332;R737.33
【正文快照】： 宫颈癌(cervical carcinoma,CC)是威胁全球女性生命健康的恶性肿瘤之一,有逐年升高的趋势,并且渐趋于年轻化[1]。近期国内外的研究表明,白介素-11(interleukin-11,IL-11)及其白介素-11受体α(interleu-kin-11 receptor,IL-11Rα)与肿瘤的发生发展密切相关,IL-11已被证明是一种，

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