细菌潜生体与盆腔脓肿发病相关性研究

发布时间：2018-02-09 11:36

本文关键词： 盆腔脓肿细菌潜生体大肠埃希菌　出处：《中国实用妇科与产科杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的探讨大肠埃希菌潜生体(CGC)的形成机制及其与盆腔脓肿发病的相关性。方法构建盆腔炎性疾病(PID)模型:以雌性SD大鼠为研究对象。开腹在肠系膜处接种大肠埃希菌,观察生殖系统的病理变化,确定PID的急性加重期。构建PID急性加重期模型:分4组,对照组1组,抗生素组3组。注射1/2浓度抗生素,模拟临床非规范化应用。切片观察病灶处病理改变,镜下观察CGC的产生。结果 SD大鼠PID模型病灶处组织病理损伤程度与时间呈正相关。模型中炎症的病理转归分为急性期与慢性期。急性加重期界定为建模后7~17 d,但均无化脓现象。构建PID急性加重期内动物模型,使用1/2浓度头孢菌素的模型组病灶出现化脓反应,组织损伤严重。脓液中检测出革兰阴性、直径3~15μm的纤细大肠埃希菌CGC。结论不规范的抗生素治疗PID可诱导CGC形成,有诱发盆腔脓肿的可能。
[Abstract]:Objective to investigate the formation mechanism of Escherichia coli and its correlation with the pathogenesis of pelvic abscess. Methods the PID-model of pelvic inflammatory disease was established in female SD rats. The acute exacerbation phase of PID was determined by observing the pathological changes of the reproductive system. The model of acute exacerbation of PID was established. The model was divided into 4 groups: control group, control group and antibiotic group. The pathological changes of the lesion were observed by biopsy. Results there was a positive correlation between the degree of pathological injury and time in the lesion of PID model of SD rats. The pathological outcome of inflammation in the model was divided into acute phase and chronic stage. The acute exacerbation stage was defined as 7d after modeling. But there was no suppuration. The animal model of acute exacerbation of PID was established. In the model group with 1/2 concentration of cephalosporin, pyogenic reaction appeared in the lesion and tissue damage was serious. Gram-negative and 3- 15 渭 m in diameter of Escherichia coli were detected in the pus. Conclusion PID can induce the formation of CGC by nonstandard antibiotic therapy. It is possible to induce pelvic abscess.
【作者单位】：哈尔滨医科大学附属二院妇产科;西安市第四医院妇产科;哈尔滨医科大学基础医学院微生物教研室;
【基金】：黑龙江省自然科学基金(H2013106)
【分类号】：R711.33

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