TLR2和TNF-α与输卵管炎的相关性研究

发布时间：2018-02-14 09:19

本文关键词： Toll样受体- 肿瘤坏死因子-α 输卵管炎　出处：《生殖医学杂志》2016年01期 　论文类型：期刊论文

【摘要】：目的建立输卵管炎模型,检测Toll样受体-2(TLR2)和肿瘤坏死因子-α(TNF-α)的表达,探讨其与输卵管炎的关系。方法 48只6~8周龄BALB/c雌性小鼠,随机平均分为空白组(不处理)和模型组(子宫底注入l×109/ml金黄色葡萄球菌悬液50μl)。分别在接种3d、7d、14d、28d处死模型组和对应时间的空白组小鼠,收集血液和输卵管。采用实时荧光定量PCR检测输卵管组织TLR2mRNA以及ELISA检测血TNF-α在模型组和相应空白组中的表达,并观察各组输卵管病理改变。结果空白组各时间点输卵管组织TLR2mRNA表达及血TNF-α浓度均无显著差异(P0.05);模型组3d、7d、14d时TLR2mRNA表达逐渐降低,TNF-α浓度逐渐升高,但均无统计学差异(P0.05),而28d时TLR2mRNA相对表达含量(0.822)显著低于3d时(1.296)(P0.05);模型组各时间点血清TNF-α水平均显著高于相应空白组(P0.05)。结论TLR2可能通过一系列信号转导,诱导产生大量TNF-α,引起输卵管炎。
[Abstract]:Objective to establish salpingitis model, detect the expression of Toll like receptor-2 (TLR2) and tumor necrosis factor- 伪 (TNF- 伪), and explore the relationship between salpingitis and salpingitis. The mice were randomly divided into blank group (untreated) and model group (50 渭 l / ml staphylococcus aureus suspension of 1 脳 10 9 / ml at the bottom of uterus). The model group and the blank group were killed on the 3rd day, 7th day, 14d and 28d, respectively. Blood and fallopian tubes were collected. The expression of TNF- 伪 in the model group and the corresponding blank group was detected by real-time fluorescence quantitative PCR and ELISA, respectively. Results there was no significant difference in the expression of TLR2mRNA and TNF- 伪 in oviducts at all time points in the blank group, while the expression of TLR2mRNA in the model group decreased gradually at the 14th day after 7 days of treatment, and the concentration of TNF- 伪 increased gradually. However, there was no significant difference in serum TNF- 伪 levels between the two groups (P 0.05), but the relative expression of TLR2mRNA at 28 days was significantly lower than that at 3 days, and the serum levels of TNF- 伪 in the model group were significantly higher than those in the control group at each time point. Conclusion TLR2 may pass through a series of signal transduction pathways, and the level of TNF- 伪 in the model group is significantly higher than that in the control group at each time point. A large amount of TNF- 伪 is induced, which causes salpingitis.
【作者单位】：金华市人民医院生殖中心;武义县中医院;浙江大学医学院附属妇产科医院中心实验室;
【基金】：金华市科技计划项目(No.2014A33503)
【分类号】：R711.76

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