胚胎监测仪延时摄像间隔时间对体外受精胚胎发育的影响

发布时间：2018-02-14 08:27

本文关键词： 胚胎监测延时摄像技术辅助生殖技术体外受精胚胎发育多细胞胚胎　出处：《山东医药》2017年03期 　论文类型：期刊论文

【摘要】：目的观察胚胎监测仪延时摄像间隔时间对体外受精胚胎发育的影响。方法选取行体外受精治疗的受试者24对,获得168个受精胚胎,随机分为A、B、C、D组,每组42个胚胎。四组胚胎均在CO_2培养箱中,在37℃、6%CO_2条件下连续培养6 d并进行观察。采用Primo vision胚胎监测仪观察各组胚胎的发育过程,A、B、C、D组的拍摄时间间隔分别为5、10、15、20 min。胚胎监测结束后,找到每个胚胎对应的监测照片和模拟视频。记录胚胎多细胞发育所需时间,观察完成受精操作60 h时胚胎发育情况,计算各组形成优质胚胎和囊胚的比例,记录各组胚胎初次分裂的卵裂模式。结果 A组胚胎发育到5细胞所需时间长于D组,A、B组发育到6、7、8细胞所需时间长于D组,A组发育到6、7、8细胞所需时间长于C组(P均0.05)。完成受精操作60 h时A组胚胎仍处在6细胞阶段,B组胚胎尚未发育到7细胞阶段,C组胚胎已分裂至7细胞阶段,D组为8细胞胚胎。C、D组形成优质胚胎、囊胚的比例均高于A组,C、D组形成囊胚的比例高于B组(P均0.05)。C、D组初次正常卵裂胚胎比例高于A组,D组正常卵裂胚胎比例高于B组,C组非轴性卵裂、非二倍性卵裂胚胎比例低于A组,C组不均一性卵裂胚胎比例高于A组,D组非轴性卵裂胚胎比例低于A组和B组(P均0.05)。结论胚胎监测仪延时摄像间隔时间对胚胎发育存在一定影响,采用15 min和20 min的拍摄间隔较5 min、10 min对胚胎发育影响更小。
[Abstract]:Objective to observe the effect of delayed camera interval of embryo monitor on the development of IVF embryos. Methods 168 fertilized embryos were obtained from 24 pairs of subjects who were treated with IVF. Each group of 42 embryos. Four groups of embryos in the CO_2 incubator, The development of embryos in each group was observed by Primo vision embryo monitor. The interval of shooting time in group D was 5 ~ 10 ~ 1520 min, respectively. The monitoring photos and simulated videos of each embryo were found. The time required for multicellular development of the embryo was recorded, the development of the embryo was observed at 60 h after the completion of the fertilization operation, and the proportion of high-quality embryos and blastocysts in each group was calculated. Results the development time from group A to 5 cells was longer than that from group A to group A from group A to group A was longer than that from group A to cell from 6 to 7 cells in group A and group A was longer than that from group C to group C. After 60 hours of fertilization, group A embryos were still in 6-cell stage, group B embryos had not developed to 7 cell stage, group C embryos had divided into 7 cell stages and group D was 8-celled embryos. Con D group formed high-quality embryos. The percentage of blastocysts in group A was higher than that in group A and group C were higher than that in group B (P = 0.05). The percentage of normal blastocyst embryos in group D was higher than that in group A and C (P = 0.05), and the proportion of normal blastocysts in group D was higher than that in group C (non-axial cleavage). The proportion of non-diploidy cleavage embryos was lower than that of group A and C, and the proportion of non-axial cleavage embryos in group A was higher than that in group A and group D. it was lower than that in group A and group B (P < 0.05). There is a certain impact on education. The effect of 15 min and 20 min interval on embryo development was less than that of 5 min and 10 min.
【作者单位】：大理大学临床医学院;大理大学第一附属医院;
【基金】：大理大学博士科研启动费项目(KYBS201612) 九三学社大理州委立项调研课题
【分类号】：R714.8

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