雌激素通过非基因效应激活钙离子流调控子宫内膜癌细胞外信号调节激酶通路

发布时间：2018-02-14 14:20

本文关键词： 子宫内膜肿瘤钙细胞外信号调节MAP激酶类雌激素类　出处：《北京大学学报(医学版)》2015年03期 　论文类型：期刊论文

【摘要】：目的:研究子宫内膜癌Ishikawa细胞中,雌激素通过钙离子通道及雌激素受体引发钙离子流继而影响细胞外信号调节激酶(extracellular signal-regulated kinases,ERK)活化通路的机制。方法:激光共聚焦实验检测雌激素作用下及加入雌激素受体的抑制剂ICI182780和钙通道阻滞剂nifedipine后胞浆内游离钙离子浓度的变化,Western-blotting方法检测同样条件下ERK通路的激活。结果:17β-雌二醇(17β-estrodiol,E2)和偶联牛血清白蛋白(bovine serum albumin,BSA)的17β-雌二醇(E2-BSA)在作用1 min后均可以引发瞬时的钙离子流,钙离子的浓度增高可以被雌激素受体的抑制剂ICI182780和钙通道阻滞剂nifedipine抑制。在Ishikawa细胞,E2可以快速激活ERK通路,加入雌激素受体抑制剂ICI182780后,E2作用5 min和30 min,ERK通路未被阻抑。加入钙离子通道阻滞剂nifedipine后,5 min时雌激素对ERK通路的激活未被阻抑,但30 min时nifedipine阻抑了ERK通路的激活。E2-BSA对ERK通路的活化也在作用30 min后被nifedipine阻抑。结论:雌激素可以通过钙离子通道快速激发钙离子流,进而影响ERK通路的活化。
[Abstract]:Objective: to study Ishikawa cells in endometrial carcinoma. Estrogen via calcium channel and estrogen receptor initiated calcium ion current and then affected extracellular signal-regulated kineses ERK activation pathway. Methods: laser confocal assay was used to detect the effects of estrogen on the activation of extracellular signal-regulated kineses (ERK). Methods: the effects of estrogen and the addition of estrogen on estrogen were detected by confocal laser. Changes in intracellular free calcium concentration after receptor inhibitor ICI182780 and calcium channel blocker nifedipine. Western-blotting method was used to detect activation of the ERK pathway under the same conditions. Results: 17 尾 -estradiol 17 尾 -estradiol E2) and 17 尾 -estradiol coupled with bovine serum albumin BSA-17 尾 -estradiol were detected by Western-blotting. E2-BSA) can induce transient calcium ion current after 1 min action. The increase of calcium concentration was inhibited by estrogen receptor inhibitor ICI182780 and calcium channel blocker nifedipine. In Ishikawa cells, E2 could rapidly activate the ERK pathway. After adding estrogen receptor inhibitor ICI1827 80, E 2 was not inhibited for 5 min and 30 minutes, but the activation of estrogen on ERK pathway was not inhibited at 5 min after the addition of calcium channel blocker nifedipine. However, at 30 min, nifedipine inhibited the activation of ERK pathway. E2-BSA also inhibited the activation of ERK pathway after 30 min. Conclusion: estrogen can stimulate Ca ~ (2 +) current through Ca ~ (2 +) channel and affect the activation of ERK pathway.
【作者单位】：北京积水潭医院妇产科;北京大学人民医院妇科;
【基金】：国家自然科学基金(30973182)资助~~
【分类号】：R737.33

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