COX-2在反复移植失败妇女种植窗期子宫内膜表达的研究

发布时间：2018-02-20 16:07

本文关键词： 反复胚胎移植失败环氧合酶-2 子宫内膜容受性　出处：《广西医科大学》2014年硕士论文　论文类型：学位论文

【摘要】：研究目的：旨在通过检测反复胚胎移植失败妇女种植窗口期子宫内膜COX-2的表达情况。探讨其与子宫内膜容受性的关系。为研究改善子宫内膜容受性及提高胚胎种植率提供理论依据。方法：选取2013年8月-12月在广西医科大学第一附属医院生殖中心就诊的不孕妇女共44例。常规接受3次或以上ET/FET均未获得临床妊娠患者16例分为A组；同期首次ET未获得妊娠患者15例分为B组；同期首次ET获得临床妊娠患者13例分为C组。应用荧光定量PCR技术检测上述不孕患者种植窗口期子宫内膜COX-2的mRNA表达情况，，探讨其与子宫内膜容受性的关系。结果：1.A.B.C三组患者一般资料的比较，如年龄、不孕年限、体重指数、不孕类型、不孕因素，受精方式，差异均无统计学意义（P＞0.05)。 2. A.B.C三组患者卵巢COH的比较及移植日子宫内膜厚度比较：基础FSH；Gn天数、Gn量，HCG日E2、P，获卵数、胚胎移植日子宫内膜厚度的比较差异无统计学意义（P＞0.05)。 3. A.B.C三组患者种植窗口期子宫内膜COX-2mRNA表达情况：3次或以上ET/FET均未获得临床妊娠患者（A组）、同期首次ET未获得妊娠患者（B组）、同期首次ET获得临床妊娠患者（C组）子宫内膜COX-2的mRNA平均相对含量分别为：A组：0.0263±0.0257，B组：0.0522±0.0309，C组：0.0598±0.0326。三组间差异有统计学意义(P＜0.05)。首次ET获得妊娠患者种植窗期子宫内膜的COX-2的mRNA的表达（C组）是反复移植失败患者（A组）的2.3倍，差异有统计学意义(P＜0.05)。首次ET未获得妊娠患者种植窗期子宫内膜的COX-2的mRNA的表达（B组）是反复移植失败患者（A组）2倍，差异有统计学意义(P＜0.05)。首次ET未获得妊娠组(B组)与首次ET获得妊娠组（C组）比较差异无统计学意义（P＞0.05）。结论：反复胚胎移植失败患者子宫内膜COX-2的mRNA平均相对表达量低于首次ET获得临床妊娠的患者。提示种植窗期子宫内膜COX-2的表达降低或缺乏，可能导致子宫内膜容受性下降，影响胚胎种植。
[Abstract]:Objective: to investigate the expression of endometrial COX-2 in women with repeated embryo transfer failure during implantation window, and to explore the relationship between endometrial receptivity and endometrial receptivity. Planting rate provides theoretical basis. Methods: a total of 44 infertile women admitted to the Reproductive Center of the first affiliated Hospital of Guangxi Medical University from August 2013 to December were selected. At the same time, 15 cases of pregnancy without et were divided into group B and 13 cases of clinical pregnancy were divided into group C. the expression of COX-2 in endometrium of the infertile patients during implantation window was detected by fluorescence quantitative PCR. To investigate the relationship between endometrial receptivity and endometrial receptivity. Results there was no significant difference in general data, such as age, years of infertility, body mass index, type of infertility, factors of infertility, and fertilization mode among the three groups (P > 0.05). 2. Comparison of ovarian COH and endometrial thickness on transplantation day in A.B.C group: there was no significant difference in endometrial thickness on embryo transfer day (P > 0.05). 3. Expression of COX-2mRNA in endometrium of three groups of A.B.C patients during implantation window period: no clinical pregnancy group (group A) was obtained for 3 times or more of ET/FET, group B was not obtained for the first time et, and group C (group C) for the first time. The average relative mRNA content of endometrial COX-2 was: 1: a group: 0.0263 卤0.0257%, group B: 0.0522 卤0.0309%, group C: 0.0598 卤0.0326.The difference between the three groups was statistically significant (P < 0.05). The expression of COX-2 in the implantation window of pregnancy patients was obtained by et for the first time (group C) was a failure of repeated transplantation. 2. 3 times as much as group A. The difference was significant (P < 0.05). The mRNA expression of COX-2 in the endometrium during implantation window stage was 2 times higher in group B than in group A, which was failed in repeated transplantation for the first time (P < 0.05). The difference was statistically significant (P < 0.05). There was no significant difference between group B (group B) and group C (group C) (P > 0.05). Conclusion: the average relative expression of mRNA in endometrial COX-2 in patients with failed repeated embryo transfer is lower than that in patients with clinical pregnancy obtained by et for the first time. It is suggested that the decrease or lack of COX-2 expression in endometrium during implantation window may lead to the decrease of endometrial receptivity. Affect embryo implantation.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R714.8

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