单核细胞趋化蛋白-1和胎膜早破相关性的研究

发布时间：2018-02-21 21:41

本文关键词： 胎膜早破单核细胞趋化蛋白-1 血清降钙素原宫颈分泌物　出处：《青岛大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:探讨孕妇宫颈分泌物中单核细胞趋化蛋白-1及血清降钙素原与胎膜早破发生的相关性,分析单核细胞趋化蛋白-1和血清降钙素原对胎膜早破发生的预测价值。方法:选择2015年1月至2016年1月在青岛市市立医院分娩和门诊产检的孕妇共120例[A组(未足月组),n=60;B组(足月组),n=60]为研究对象根据胎膜早破发生的孕周:孕28~36+6周和孕≥37周,分为未足月胎膜早破组(A1组,n=30)和足月胎膜早破组(B1组,n=30);相对应筛选同期正常未临产孕妇为正常对照组,分为未足月健康对照组(A0组,n=30)和足月健康对照组(B0组,n=30)。采用定量双抗体夹心酶联免疫吸附(ELISA)法测定宫颈分泌物MCP-1水平,并测定血清降钙素原含量。各组数据计量资料以(x?s)采用t检验进行统计学处理,P≤0.05为具有统计学意义。结果:各组间年龄和未足月胎膜早破与足月胎膜早破间的绒毛膜羊膜炎发生率并无明显差异(20%和13.3%,P0.05)。宫颈分泌物MCP-1水平的比较:A1组A0组(70.86±12.76 pg/ml16.64±2.97pg/ml,P0.01),两组差异具有显著的统计学意义;B1组B0组(30.26±5.22pg/ml13.19±2.35pg/ml,P0.05),两组差异具有统计学意义;A1组B1组(70.86±12.76pg/ml30.26±5.22pg/ml,P0.01),两组差异具有显著的统计学意义。血清降钙素原水平的比较:A1组A0组(0.95±0.32ng/ml0.35±0.16ng/ml,P0.05),两组差异具有统计学意义;B1组B0组(0.25±0.06ng/ml0.05±0.02ng/ml,P0.05),两组差异具有统计学意义;A1组B1组(0.95±0.32ng/ml0.25±0.06ng/ml,P0.05),两组差异具有统计学意义。结论:1.胎膜早破孕妇中宫颈分泌物MCP-1水平较正常对照组明显升高,提示宫颈分泌物MCP-1水平的升高与胎膜早破相关;未足月胎膜早破宫颈分泌物中MCP-1水平明显高于其他组,推测宫颈分泌物中MCP-1水平升高可能与未足月胎膜早破的关系更密切。2.推测宫颈分泌物MCP-1水平可作为预测未足月胎膜早破发生的指标。3.未足月胎膜早破孕妇血清降钙素原明显升高,推测血清降钙素原水平在预测未足月胎膜早破中更为敏感。4.宫颈分泌物MCP-1水平和血清降钙素原水平的联合检测或许对未足月胎膜早破的预测具有意义。
[Abstract]:Objective: to investigate the relationship between the occurrence of premature rupture of membranes and monocyte chemoattractant protein-1 and serum procalcitonin in cervical secretion of pregnant women. To analyze the predictive value of monocyte chemoattractant protein-1 and serum procalcitonin on premature rupture of membranes. Methods: 120 pregnant women who gave birth and outpatient delivery in Qingdao City Hospital from January 2015 to January 2016 were selected. According to the gestational weeks of premature rupture of membranes, the gestational weeks were 2836 weeks of gestation and 37 weeks of pregnancy. They were divided into two groups: group A 1 (group A 1) and group B 1 (group B 1). MCP-1 level of cervical secretion and serum procalcitonin content were measured by quantitative double antibody sandwich enzyme linked immunosorbent assay (Elisa) and calcitonin content in serum were measured by quantitative double antibody sandwich enzyme linked immunosorbent assay (Elisa). Results: there was no significant difference in age and the incidence of chorioamnionitis between preterm premature rupture of membranes and premature rupture of full-term membranes in all groups (P < 0.05). There was a significant difference between the two groups (30.26 卤5.22pg / ml 13.19 卤2.35pg / ml P 0.05). The difference between the two groups was statistically significant. The difference between the two groups was statistically significant. The difference between the two groups was statistically significant. The serum calcitonin levels in group A1 were 70.86 卤12.76pg / ml 30.26 卤5.22pgml / ml respectively. There was a significant difference in serum calcitonin between the two groups. Comparison of the original level: 0.95 卤0.32ng / ml 0.35 卤0.16ng / ml P0.05A, there is significant difference between the two groups. Conclusion there is a significant difference between the two groups in the cervical secretion of pregnant women with premature rupture of membranes (0.25 卤0.06ng / ml 0.05 卤0.02ng / ml P 0.05), and 0.95 卤0.32ng / ml 0.25 卤0.06ng / ml P0.05U in the A1 group. Conclusion there is a significant difference between the two groups in the secretion of cervix in pregnant women with premature rupture of membranes. The level of MCP-1 was significantly higher than that of the normal control group. The results suggest that the increase of MCP-1 level in cervical secretion is related to premature rupture of membranes, and the level of MCP-1 in unmatured premature rupture of cervical membrane is significantly higher than that in other groups. It is speculated that the increase of MCP-1 level in cervical secretion may be more closely related to premature rupture of unmatured membranes. 2. Speculating that the level of MCP-1 in cervical secretion can be used as an index to predict the occurrence of premature rupture of unmatured membranes. 3. The serum level of pregnant women with premature rupture of unfull-term membranes may be decreased. The level of calcitonin was significantly increased. It is speculated that serum procalcitonin level is more sensitive in predicting premature rupture of unmatured membranes. Combined detection of MCP-1 level in cervical secretion and serum procalcitonin level may be of significance for prediction of preterm premature rupture of membranes.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R714.433

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