IVF中未见原核卵裂胚胎囊胚滋养层细胞的CNV检测价值研究

发布时间：2018-02-27 23:23

本文关键词： 未见原核卵裂胚胎囊胚拷贝数变异　出处：《中国现代医学杂志》2017年09期 　论文类型：期刊论文

【摘要】：目的对常规体外受精(IVF)中未见原核卵裂胚胎囊胚滋养层细胞10 MB拷贝数变异进行分析。方法对IVF受精的未见原核卵裂胚胎囊胚培养,利用全基因组测序的方法对D5评级3BC囊胚的滋养层细胞进行10MB的拷贝数变异检测,判断未见原核卵裂胚胎囊胚非整倍体变异程度。结果共选择未见原核卵裂胚胎69例,形成囊胚51例(73.91%);囊胚评级3BC者共33例,对其进行滋养层细胞活检并进行拷贝数变异检测,检测成功23例(70%),其中正常核型(46,XN)17例(73.9%),异常6例(26.1%)。结论未见原核卵裂胚胎经囊胚培养可以获得较高比例染色体正常胚胎,结合胚胎植入前遗传学诊断及产前诊断技术,未见原核卵裂囊胚具有利用价值。
[Abstract]:Objective to analyze the variation of 10 MB copy number in trophoblast cells of prokaryotic cleavage embryo in vitro fertilization (IVF). Methods to culture the blastocyst without prokaryotic cleavage in IVF fertilization. A total of 10 MB copy number variation was detected in trophoblast cells of D5 rated 3BC blastocysts by whole genome sequencing, and the degree of aneuploidy of blastocysts without prokaryotic cleavage was determined. Results 69 non-prokaryotic blastocysts were selected. There were 51 cases of blastocyst and 33 cases of blastocyst with 3BC grade. The trophoblastic cell biopsy was performed and copy number variation was detected. 23 cases were detected successfully, of which 17 cases were normal karyotype 46 X Ns, and 6 cases were abnormal. Conclusion A high proportion of normal chromosomal embryos can be obtained by blastocyst culture in prokaryotic cleavage embryos, combined with genetic diagnosis and prenatal diagnosis techniques before embryo implantation. No prokaryotic blastocysts were found to be useful.
【作者单位】：内蒙古医科大学附属医院生殖中心;
【基金】：内蒙古自然科学基金(No:2015BS0802) 内蒙古教育厅基金(No:NJZY16118) 内蒙古医科大学科技百万工程(No:YKD2014KJBW007、YKD2015KJBW023)
【分类号】：R714.8

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