抗苗勒管激素蛋白在成年小鼠卵泡的生长发育及卵巢衰老发生发展中的调控作用

发布时间：2018-02-28 08:02

本文关键词： 抗苗勒管激素卵巢衰老颗粒细胞　出处：《中国老年学杂志》2015年18期 　论文类型：期刊论文

【摘要】：目的探讨抗苗勒管激素蛋白在成年小鼠卵泡的生长发育调控及卵巢衰老发生发展中的调控作用。方法分别取12、24、36、48 w的SPF级雌性C57小鼠各20只。收集12周龄小鼠不同发育阶段的卵巢以及24、36、48周龄自然衰老小鼠卵巢,采用免疫组化法和Western印迹技术检测各年龄段卵巢中抗苗勒管激素蛋白的表达。结果抗苗勒管激素蛋白表达水平随着卵泡发育时间延长而逐渐提升,在始基卵泡、初级卵泡、次级卵泡、窦前卵泡细胞中均有表达,但在闭锁卵泡、黄体颗粒、卵母细胞中无表达,在窦前卵泡细胞中表达水平最高。不同年龄小鼠卵巢组织的卵泡和间质中均存在有抗苗勒管激素蛋白表达,且卵泡颗粒细胞中表达水平无明显差异,但随着鼠龄增加,卵巢间质细胞中抗苗勒管激素蛋白表达水平逐渐提升。抗苗勒管激素蛋白在间质中表达水平在不同鼠龄间差异有统计学意义(P0.05);12周龄小鼠表达水平明显低于其他周龄(P0.01);24周龄、36周龄小鼠表达水平均明显低于48周龄小鼠(P0.05);24周龄、36周龄小鼠表达水平之间差异无统计学意义(P0.05)。Western印迹检测显示,随着鼠龄的增加抗苗勒管激素蛋白在小鼠整个卵巢组织中的表达呈逐渐降低的趋势(P0.05)。结论抗苗勒管激素蛋白主要表达于卵泡发育早期阶段的颗粒细胞,其在间质中随鼠龄增加表达逐渐提升,但在整个卵巢组织中随鼠龄增加表达逐渐下降,说明抗苗勒管激素蛋白在卵泡发育和卵巢老化发生发展中起到一定的调控作用。
[Abstract]:Objective to investigate the role of anti-mullerian hormone protein in the regulation of follicle growth and development and the development of ovarian senescence in adult mice. The ovaries of the developmental stage and the ovaries of the natural aging mice at the age of 24 ~ 36 ~ (48) weeks, Immunohistochemical method and Western blotting technique were used to detect the expression of anti-Mullerian tube hormone protein in ovary of all ages. Results the expression level of anti-Mullerian tube hormone protein increased gradually with the development of follicle, in the primary follicle and primary follicle, the expression level of anti-Muller tube hormone protein increased gradually with the development of follicle. Secondary follicles and preantral follicular cells were expressed, but no expression was found in atretic follicles, luteal granules and oocytes. The highest expression level was found in preantral follicle cells. The expression of anti-mullerian hormone protein was found in follicles and stroma of different age mice, and there was no significant difference in the expression level of granulosa cells in follicular granulosa cells, but the expression level increased with the age of mice. The expression level of anti-mullerian hormone protein in stromal cells of ovary increased gradually, and the expression level of anti-mullerian hormone protein in interstitial tissue was significantly lower than that in 12-week-old mice at 12 weeks of age. There was no significant difference in the expression level of P0.01, P0.01-, 24-week-old and 36-week-old mice compared with that of the 48-week-old mice. Western blot analysis showed that there was no significant difference in the expression levels of P0.05 and P0.05. Western blot analysis showed that the expression level of P0.05 was significantly lower than that of the 48-week-old mice. The expression of anti-mullerian hormone protein in mouse ovary tissue decreased gradually with the increase of mouse age. Conclusion the anti-mullerian hormone protein is mainly expressed in granulosa cells at early stage of follicular development. Its expression increased gradually with the increase of mouse age, but decreased gradually in the whole ovarian tissue, indicating that the anti-mullerian hormone protein plays a role in regulating follicular development and ovarian aging.
【作者单位】：西安医学院护理学院;西安医学院第二附属医院;
【分类号】：R711.75

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