BML-111下调骨桥蛋白对脂多糖诱导的Hela细胞增殖的影响及其机制

发布时间：2018-03-04 05:08

本文选题：宫颈肿瘤　切入点：脂氧素　出处：《实用医学杂志》2015年03期 　论文类型：期刊论文

【摘要】：目的:研究脂氧素类似物BML-111对脂多糖(lipopolysaccharide,LPS)诱导的宫颈癌细胞株Hela细胞增殖及骨桥蛋白(osteopontin,OPN)表达的影响。方法:分别用100、10、1、0.1μg/m L LPS刺激Hela细胞,MTT方法检测其对细胞存活率的影响;Western blot免疫印迹法检测LPS对Hela细胞OPN及p53蛋白表达的影响。Hela细胞分4组:(1)空白组;(2)LPS组;(3)LPS+BML-111组;(4)LPS+BML-111+Boc-2(BML-111受体FPR2/ALX的阻断剂)组。MTT方法检测BML-111对LPS诱导的Hela细胞存活率的影响;Western blot免疫印迹法检测BML-111对LPS诱导的Hela细胞OPN及p53(增殖指标)蛋白表达的影响。结果:与对照组相比,100、10、1、0.1μg/m L LPS均能明显促进Hela增殖及OPN、p53蛋白表达(P0.05),但不同浓度组间比较差异无显著性(P0.05)。BML-111能明显抑制LPS诱导的Hela细胞存活率,并能抑制OPN及p53蛋白的表达,Boc-2能阻断这种效应。结论:BML-111能抑制LPS诱导Hela细胞增殖,其作用可能是通过与受体结合,进而抑制OPN下调p53实现。
[Abstract]:Objective: to study the effects of lipoxygenin analogues (BML-111) on the proliferation and osteopontin expression of cervical cancer cell line Hela induced by lipopolysaccharide (LPS). Methods: the survival rate of Hela cells was measured by using 100 渭 g / ml LPS and 0.1 渭 g / mL LPS. Effect of LPS on the expression of OPN and p53 protein in Hela cells by Western blot Western blotting. Hela cells were divided into 4 groups. The effect of BML-111 on the expression of OPN and p53 protein in Hela cells induced by LPS was detected by Western blot Western blot. Results: compared with the control group, the expression of OPN and p53 protein in Hela cells induced by LPS was significantly increased by 0.1 渭 g / mL LPS, but at different concentrations. There was no significant difference in the survival rate of Hela cells induced by LPS. Boc-2 can inhibit the expression of OPN and p53 protein. Conclusion: BML-111 can inhibit the proliferation of Hela cells induced by LPS, which may be achieved by binding to the receptor and then inhibiting the down-regulation of p53 by OPN.
【作者单位】：南昌大学第二附属医院病理科;南昌大学第二附属医院特需病房;
【基金】：国家青年科学基金项目(编号:81301741) 江西省青年科学基金项目(编号:20122BAB215007) 江西省自然科学基金项目(编号:20132BAB205041) 江西省教育厅青年基金项目(编号:GJJ13037)
【分类号】：R737.33

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