放化疗联合DR5单克隆抗体对人卵巢癌Caov-3细胞凋亡的影响

发布时间：2018-03-05 13:24

本文选题：DR　切入点：单克隆抗体　出处：《广东医学》2015年19期 　论文类型：期刊论文

【摘要】：目的探讨抗人DR5单克隆抗体联合放化疗诱导卵巢癌Caov-3细胞凋亡的机制。方法常规培养人卵巢癌细胞系Caov-3,流式细胞术定量分析检测Caov-3细胞表面DR5的表达。通过细胞组织化学方法分析DR5在细胞的分布情况。Annexin V-FITC/PI双色标记Caov-3细胞,流式细胞术定量分析检测细胞凋亡率;在共聚焦显微镜下观察抗人DR5单克隆抗体对Caov-3细胞形态变化的影响。共聚焦显微镜检测ΔΨm,MTT法检测抗DR5单克隆抗体、顺铂、60Co放射线对细胞增殖的抑制活性。结果 Caov-3细胞表面有DR5表达,其平均表达百分率为(47.5±4.3)%,DR5分布在细胞质并靠近细胞核的位置。Hoechst染色证实杀伤作用是通过细胞凋亡实现的,经流式细胞术检测显示,10μg/L抗人DR5单克隆抗体的作用Caov-3细胞24 h导致20.0%的细胞发生凋亡;在共聚焦显微镜下可观察到诱导导致Caov-3细胞呈现典型细胞凋亡的形态特征。经Rhodamine123荧光染色之后,通过共聚焦显微镜可观察到胞浆是荧光主要存在的部位,而线粒体中部分荧光消失。抗体与顺铂和放射线联合作用对肿瘤细胞株的细胞毒作用增强。结论抗人DR5单克隆抗体能够诱导人卵巢癌细胞系Caov-3凋亡。抗体与DDP和放射线联合作用可增强对肿瘤细胞株的细胞毒作用。
[Abstract]:Objective to investigate the mechanism of apoptosis induced by anti-human DR5 monoclonal antibody combined with radiotherapy and chemotherapy in human ovarian cancer Caov-3 cell line Caov-3.Methods the expression of DR5 on the surface of human ovarian cancer cell line Caov-3 was quantitatively detected by flow cytometry. The distribution of DR5 in cells was analyzed by histochemical method. Annexin V-FITC / Pi double color labeled Caov-3 cells. Apoptosis rate was detected quantitatively by flow cytometry, the effect of anti-human DR5 monoclonal antibody on the morphological changes of Caov-3 cells was observed under confocal microscope, and the monoclonal antibody against DR5 was detected by confocal microscopy. Results there was DR5 expression on the surface of Caov-3 cells. The average expression percentage of DR5 was 47.5 卤4.3%. The killing effect was confirmed by apoptosis in the cytoplasm and near the nucleus. Flow cytometry showed that 10 渭 g / L monoclonal antibody against human DR5 induced apoptosis in 20.0% Caov-3 cells for 24 h. The morphological characteristics of typical apoptosis of Caov-3 cells were observed under confocal microscope. After Rhodamine123 fluorescence staining, the cytoplasm was observed to be the main site of fluorescence. The cytotoxic effect of antibody combined with cisplatin and radiation on tumor cell line was enhanced. Conclusion Anti-human DR5 monoclonal antibody can induce apoptosis of human ovarian cancer cell line Caov-3. The combination of radiation and radiation can enhance the cytotoxicity of tumor cell line.
【作者单位】：天津中医药大学第一附属医院老年病科;
【分类号】：R737.31

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