miR-425对卵巢癌细胞迁移和侵袭的影响及其机制

发布时间：2018-03-09 05:20

本文选题：卵巢肿瘤　切入点：miR-　出处：《山东医药》2016年45期 　论文类型：期刊论文

【摘要】：目的探讨miR-425对卵巢癌细胞迁移和侵袭的影响及其机制。方法 RT-PCR测定卵巢细胞系SKOV3及正常卵巢细胞系HOSE中miR-425表达水平,将SKOV3细胞分为lent-shmiR-425和lent-shvector组,分别转染lent-shmiR-425和lent-shvector,24 h后行细胞划痕试验和Transwell侵袭小室试验。应用Target Scan预测miR-425与PTEN因子3'端非翻译区结合位点。将SKOV3细胞分成3组:野生型质粒组、突变型质粒组、阴性对照组,分别共转染miR-425及野生型荧光素酶报告质粒p GL3-PTEN 3'UTR,miR-425和突变型荧光素酶报告质粒p GL3-PTEN 3'WT,miR-425和对照荧光素酶质粒,用荧光素酶试验检测各组荧光素酶活性。结果在卵巢癌细胞系SKOV3中miR-425相对表达量为2.1,正常卵巢细胞系HOSE为1.0,两者比较,P0.05。lent-shmiR-425组划痕愈合率为27.56%±2.14%,lent-shvector组划痕愈合率为89.15%±6.24%,两组比较,P0.05。lent-shmiR-425组侵袭细胞数为(75±10)个/200倍视野,lent-shvector组侵袭细胞数为(160±20)个/200倍视野,两组比较,P0.05。Target Scan预测miR-425与PTEN因子3'端非翻译区的结合位点结合;野生型质粒组、突变型质粒组、阴性对照组荧光素酶活性分别为23.70±0.93、99.27±5.90、104.00±4.02。野生型质粒组低于突变型质粒组和阴性对照组(P均0.05)。突变型质粒组与阴性对照组比较,P0.05。结论 miR-425水平升高可促进卵巢癌细胞迁移和侵袭,其机制可能与miR-425靶向调节PTEN表达有关。光素酶活性分别为23.70±0.93、99.27±5.90、104.00±4.02。野生型质粒组低于突变型质粒组和阴性对照组(P均0.05)。突变型质粒组与阴性对照组比较,P0.05。结论 miR-425水平升高可促进卵巢癌细胞迁移和侵袭,其机制可能与miR-425靶向调节PTEN表达有关。
[Abstract]:Objective to investigate the effect of miR-425 on migration and invasion of ovarian cancer cells and its mechanism. Methods the expression of miR-425 in ovarian cell line SKOV3 and normal ovarian cell line HOSE was determined by RT-PCR. SKOV3 cells were divided into lent-shmiR-425 and lent-shvector groups. After transfection of lent-shmiR-425 and lent-shvectorus for 24 h, cell scratch test and Transwell invasion chamber test were performed. Target Scan was used to predict the 3'untranslated site of miR-425 and PTEN factor. SKOV3 cells were divided into three groups: wild-type plasmid group, mutant plasmid group and negative control group. MiR-425 and wild-type luciferase reporter plasmids p GL3-PTEN _ 3 UTR-miR-425, mutant luciferase reporter plasmids p GL3-PTEN _ 3 ~ + WTT ~ + miR-425 and control luciferase plasmids were cotransfected, respectively. Luciferase test was used to detect luciferase activity in each group. Results the relative expression of miR-425 was 2.1 in ovarian cancer cell line SKOV3 and 1.0 in normal ovarian cell line HOSE. The rate of scratch healing of P0.05.lent-shmiR-425 group was 27.56% 卤2.14% and the rate of scratch healing was 89.15% 卤6.24 in the two groups. The number of invading cells in P0.05.lent-shmiR-425 group was 75 卤10) and the number of invading cells in LHS vector group was 160 卤20%. P0.05. Target Scan was used to predict the binding site of miR-425 to 3 'untranslated region of PTEN factor, wild type plasmid group, mutant plasmid group, wild type plasmid group, mutagenesis plasmid group, 3' -terminal untranslated region of PTEN factor. The luciferase activity in the negative control group was 23.70 卤0.93 卤99.27 卤5.90 卤104.00 卤4.02 respectively. The level of miR-425 in the wild type plasmid group was lower than that in the mutant plasmid group and the negative control group (P < 0.05). Conclusion the increase of miR-425 level can promote the migration and invasion of ovarian cancer cells. The mechanism may be related to the targeting regulation of PTEN expression by miR-425. The photoenzyme activity is 23.70 卤0.93 卤99.27 卤5.90,104.00 卤4.02 respectively. The level of miR-425 in the wild type plasmid group is lower than that in the mutant plasmid group and the negative control group (P 0.05). Conclusion the level of miR-425 in the mutant plasmid group is higher than that in the negative control group. Elevated ovarian cancer can promote migration and invasion of ovarian cancer cells. The mechanism may be related to the targeting regulation of PTEN expression by miR-425.
【作者单位】：武汉市武昌医院;
【分类号】：R737.31

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