PP2A抑制剂对宫颈癌细胞Ca ski辐射敏感性的影响

发布时间：2018-03-16 21:50

本文选题：蛋白磷酸酶A(PPA)　切入点：宫颈癌　出处：《华中科技大学学报(医学版)》2017年03期 　论文类型：期刊论文

【摘要】：目的探索蛋白磷酸酶2A(PP2A)抑制剂LB-100对宫颈癌细胞Ca ski辐射敏感性的影响。方法 Ca ski(宫颈癌细胞株)及CCD 841CoN(正常小肠上皮细胞株)给予不同浓度梯度(0~100μmol/L)LB-100处理24h后,以CCK-8法检测细胞活性,筛选最佳药物作用浓度。在此药物浓度下,以对照(PBS)、单纯LB-100、单纯辐照及LB-100联合辐照等方式处理细胞(辐照剂量为0、2、4、6Gy),通过平板克隆实验分别观察LB-100对Ca ski和CCD 841CoN辐射敏感性的影响;再以相同的方式处理(细胞辐照剂量为6Gy),通过流式细胞术检测凋亡及细胞周期,初探LB-100增强Ca ski辐射敏感性的机制。结果 LB-100对Ca ski及CCD 841CoN的抑制作用呈药物剂量依赖性;2.5μmol/L LB-100增强Ca ski细胞辐射敏感性,但对CCD 841CoN的辐射敏感性无显著影响;LB-100联合辐照处理促进Ca ski细胞凋亡,LB-100联合辐照组细胞凋亡率与单纯辐照组比较差异有统计学意义[(8.76±1.02)%vs.(4.65±0.72)%,P0.01];LB-100可下调Ca ski细胞G0/G1期所占比例,LB-100联合辐照组与单纯辐照组比较差异有统计学意义[(19.87±2.54)%vs.(30.73±1.18)%,P0.05]。结论 PP2A抑制剂LB-100通过促进凋亡、影响细胞周期,增强了人宫颈癌细胞株Ca ski的辐射敏感性,同时,其对正常肠上皮细胞CCD 841CoN无辐射增敏作用,有望成为临床上增强宫颈癌放射治疗疗效的辅助药物。
[Abstract]:Objective to investigate the effect of protein phosphatase 2Am PP2A inhibitor (LB-100) on the radiosensitivity of cervical cancer cells to Ca ski radiation. Methods Ca LB-100 (cervical carcinoma cell line) and CCD 841 CoN (normal small intestinal epithelial cell line) were treated with different concentration gradient 100 渭 mol/L)LB-100 for 24 h. CCK-8 assay was used to detect cell activity and to screen the best drug concentration. The effects of LB-100 on the radiosensitivity of Ca ski and CCD 841CoN were observed by plate clone assay. The cells were treated with PBSX, LB-100, single irradiation and combined irradiation with LB-100 (irradiation dose of 0 ~ 2 ~ 2 ~ 4 ~ 4) Gy ~ (6) ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1); Then treated in the same way (cell irradiation dose was 6 Gy), apoptosis and cell cycle were detected by flow cytometry. Results the inhibitory effects of LB-100 on Ca ski and CCD 841 can increase the radiosensitivity of Ca ski cells in a dose-dependent manner (2.5 渭 mol/L LB-100). But the radiosensitivity of CCD 841CoN was not significantly affected by LB-100 combined with irradiation. There was a significant difference in apoptosis rate between LB-100 and irradiation group [8.76 卤1.02 vs 4.65 卤0.72% P0.01] LB-100 could down-regulate the ratio of G _ 0 / G _ 1 phase of Ca ski cells to G _ 0 / G _ 1 phase in LB-100 + irradiation group [8.76 卤1.02 vs 4.65 卤0.72] LB-100 could down-regulate the ratio of G _ 0 / G _ 1 phase in Ca ski cells. There was statistically significant difference between the LB-100 combined irradiation group and the single irradiation group [19.87 卤2.54 vs 30.73 卤1.18]. Conclusion LB-100, a PP2A inhibitor, promotes apoptosis by promoting apoptosis. The cell cycle was affected and the radiosensitivity of human cervical cancer cell line Ca ski was enhanced. At the same time, it had no radiosensitizing effect on normal intestinal epithelial cell line CCD 841CoN, which is expected to be an auxiliary drug to enhance the therapeutic effect of radiotherapy for cervical cancer.
【作者单位】：第三军医大学护理学院;第三军医大学预防医学院全军复合伤研究所(创伤烧伤与复合伤国家重点实验室);
【分类号】：R737.33

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