原发性卵巢功能不全小鼠卵巢血流与性激素浓度相关性研究

发布时间：2018-03-17 13:54

本文选题：原发性卵巢功能不全　切入点：超声血流　出处：《首都医科大学学报》2017年06期 　论文类型：期刊论文

【摘要】：目的研究原发性卵巢功能不全(primary ovarian insufficiency,POI)小鼠卵巢的血流动力学与性激素浓度的相关性。方法以小鼠透明带3作为抗原,皮下多点注射,免疫BALB/C雌性小鼠建立原发性卵巢功能不全模型,利用超声动态观察卵巢的形态,同时利用超声彩色多普勒成像观察卵巢的血流变化,最后取小鼠血清进行性激素浓度检测。结果超声下可观察到模型组小鼠卵巢体积减小,卵泡数目减少,血流分布较弱。与空白组小鼠相比较,模型组小鼠阻力指数(resistive index,RI)较高,搏动指数(pulsatility index,PI,PI=S-D/Vmean)较低,脉压差(pulse pressure,PP)呈升高趋势;与空白组小鼠相比较,模型组小鼠的促卵泡生长激素(follicle-stimulating hormone,FSH)、抗苗勒管激素(anti-Müllerian hormone,AMH)均差异有统计学意义(P0.05)。进一步通过模型组小鼠血流动力学与激素浓度相关性检验,发现模型组小鼠PP与FSH有一定(P0.05)。结论 POI小鼠血流动力学PP与血清学FSH浓度有显著相关性,本研究为动态观察POI治疗过程提供参考。
[Abstract]:Objective to study the correlation between ovarian hemodynamics and sex hormone concentration in primary ovarian efficient Poi mice. Methods the mouse pellucida 3 was used as antigen, and was injected subcutaneously with multiple sites. The primary ovarian insufficiency model was established by immunizing BALB/C female mice. The morphology of ovary was observed dynamically by ultrasound, and the changes of ovarian blood flow were observed by color Doppler imaging. Results the ovarian volume, follicle number and blood flow distribution were decreased in the model group. Compared with the control group, the resistance index (RI) of the model group was higher than that of the control group. The pulsatility index (Pi) was lower and the pulse pressure pressure difference (PPP) was higher than that in the blank group (P < 0.05), and compared with the control group, the pulsatility index was lower, and the pulse pressure pressure index (PP) was higher than that in the control group. There were significant differences in follicle-stimulating hormone follicle-stimulating hormone (FSH) and anti-M 眉 llerian hormone (llerian) between the model group and the control group (P 0.05). It was found that PP and FSH in the model group had a certain P0.050.Conclusion there is a significant correlation between hemodynamic PP and serological FSH concentration in POI mice. This study provides a reference for dynamic observation of the course of POI treatment.
【作者单位】：首都医科大学基础医学院;首都医科大学中医药学院;
【基金】：北京市自然科学基金面上项目(7162026) 北京中医药薪火传承“3+3”工程项目(2012-SZ-C-41) 首都中医药科技专项(JJ2013-01) 首都医科大学中医药专项课题(15ZY05)~~
【分类号】：R-332;R711.75

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