生长分化因子-9和骨形成蛋白-15对多囊卵巢综合征患者卵丘细胞凋亡的影响

发布时间：2018-03-17 14:51

本文选题：生长分化因子-(GDF-)　切入点：骨形成蛋白-(BMP-)　出处：《中华生殖与避孕杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的探讨控制性超促排卵(COH)周期多囊卵巢综合征(PCOS)患者生长分化因子-9(GDF-9)、骨形成蛋白-15(BMP-15)的表达对卵丘细胞凋亡的影响。方法收集本院行卵胞质内单精子注射(ICSI)治疗的PCOS组(n=30)和对照组(n=60)患者的卵丘细胞。TUNEL法及流式细胞术检测卵丘细胞凋亡;免疫组织化学方法检测卵丘细胞GDF-9、BMP-15蛋白表达,运用Motic Med 6.0数码医学图像分析系统对染色强度定量分析;Real-time PCR技术检测卵丘细胞GDF-9、BMP-15、B细胞淋巴瘤/白血病-2(BCL)-2、BCL-2相关X蛋白(BAX)mRNA表达。结果 PCOS组穿刺卵泡数、获卵数高于对照组(P0.01);PCOS组卵丘细胞凋亡率高于对照组(P0.05);GDF-9、BMP-15蛋白在PCOS组和对照组卵丘细胞胞质中均呈阳性表达;PCOS患者卵丘细胞GDF-9、BMP-15、BCL-2、BAX mRNA的表达低于对照组(P0.05),BAX/BCL-2比值高于对照组(P0.05)。Pearson相关分析结果显示PCOS患者GDF-9、BMP-15与BCL-2 mRNA表达呈正相关,与BAX mRNA的表达呈负相关。结论 PCOS患者卵丘细胞GDF-9、BMP-15异常表达可能是卵丘细胞凋亡增加的原因之一。
[Abstract]:Objective to investigate the effect of the expression of growth differentiation factor (GDF-9) and bone morphogenetic protein-15 (BMP-15) on the apoptosis of cumulus cells in patients with polycystic ovary syndrome (PCOSs) during the controlled hyperstimulation of ovulation (COH) cycle. The apoptosis of cumulus cells was detected by Tunel method and flow cytometry in PCOS group (n = 30) and control group (n = 60). Immunohistochemical method was used to detect the expression of GDF-9 BMP-15 protein in cumulus cells. Motic Med 6.0 digital medical image analysis system was used to quantitatively analyze the staining intensity and real-time PCR technique was used to detect the expression of BDF-9BMP-15B cell lymphoma / Leukemia BCL-2BCL-2 associated X protein. Results the number of punctured follicles in the PCOS group was determined. The apoptotic rate of cumulus cells in the control group was higher than that in the control group. The expression of GDF-9BMP-15 protein in the cytoplasm of the cumulus cells in PCOS and control group was higher than that in the control group. The expression of GDF-9BMP-15 BCL-2BAX mRNA in the cumulus cells of PCOS patients was lower than that in the control group (P 0.05) and the ratio of Bax / BCL-2 in the control group was higher than that in the control group. The expression of BMP-15 in patients with PCOS was positively correlated with the expression of BCL-2 mRNA. Conclusion the abnormal expression of BMP-15 in cumulus cells of PCOS patients may be one of the reasons for the increase of apoptosis of cumulus cells.
【作者单位】：福建医科大学附属福建省妇幼保健院;厦门大学附属第一医院生殖医学中心;
【基金】：福建省临床重点专科建设项目(闽卫科教2012149) 福建省自然科学基金项目(2013J01267) 福建医科大学附属福建省妇幼保健院科研项目(15-06)~~
【分类号】：R711.75

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