酪氨酸激酶受体A在子宫腺肌病患者子宫结合带中的表达及其与痛经的相关性研究

发布时间：2018-03-18 18:03

本文选题：子官腺肌病　切入点：子宫结合带　出处：《浙江大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的：研究酪氨酸激酶受体A(tyrosine kinase receptor A, TrkA)在子宫腺肌病(Adenomyosis, AM)患者子宫结合带(the uterine junctional zone, JZ)中的表达及其与痛经和血清CA125水平的关系,探讨TrkA与子宫腺肌病痛经的关系。方法：收集24例因子宫腺肌病行子宫全切或次全切除的病例作为研究组(AM组),其中处于子宫内膜增生期15例,分泌期9例；收集同期因子宫肌瘤行子宫全切或次全切除的病例作为对照组,其中处于子宫内膜增生期9例,分泌期6例。术前通过视觉模拟评分法(visual analogue scale/score, VAS)进行疼痛程度评分,用免疫组化SP法检测两组子宫内膜和JZ组织中TrkA的表达,比较JZ组织中增生期与分泌期TrkA的表达差异,分析其与痛经的关系,及与血清CA125水平的相关性。结果： 1.TkrA在子宫内膜及JZ组织中均有表达。 2.TrkA在AM组内膜和JZ中的表达均高于对照组,差异具有统计学意义(P0.05)；TrkA在AM组和对照组JZ中的表达均高于内膜,差异具有统计学意义(P0.05)。 3.在子宫内膜增生期与分泌期,TrkA在子宫腺肌病患者的JZ中均可见表达,在增生期的表达高于分泌期,且差异具有统计学意义(P0.05)。 4.在AM组JZ中,随着疼痛程度的增加,TrkA的表达逐渐增加,两者具有显著正相关性(r=0.571,P0.05)。 5.在AM组JZ中,随着血清CA125水平的增加,TrkA的表达逐渐增加,两者具有显著正相关性(r=0.678,P0.05)。结论：子宫腺肌病中TrkA的表达明显增加,且与疼痛程度呈正相关,提示TrkA可能参与子宫腺肌病痛经的发生。
[Abstract]:Objective: to study the expression of tyrosine kinase receptor A (TrkA) in the uterine junctional zonein of adenomyosis and its relationship with the levels of dysmenorrhea and serum CA125, and to explore the relationship between TrkA and dysmenorrhea in adenomyosis. Methods: Twenty-four patients with adenomyosis underwent total or subtotal hysterectomy as study group. 15 cases were in endometrial hyperplasia and 9 cases were in secretory phase. The patients who underwent hysterectomy or subtotal hysterectomy during the same period were collected as the control group, including 9 cases in endometrial hyperplasia and 6 cases in secretory phase. The pain degree was evaluated by visual analogue scale / score (vas) before operation. Immunohistochemical SP method was used to detect the expression of TrkA in endometrium and JZ tissues. The difference of TrkA expression in proliferative phase and secretory phase was compared, and the relationship between TrkA expression and dysmenorrhea and serum CA125 level was analyzed. Results:. 1. TkrA was expressed in both endometrium and JZ tissues. 2. The expression of TrkA in the endometrium and JZ of AM group was higher than that in the control group, and the difference was statistically significant (P 0.05). The expression of TrkA in JZ of AM group and control group was higher than that of the control group, and the difference was statistically significant. 3. TrkA expression was found in both proliferative and secretory stages of endometrium in patients with adenomyosis. The expression of TrkA in proliferative phase was higher than that in secretory phase, and the difference was statistically significant (P 0.05). 4. In AM group, the expression of TrkA increased with the increase of pain, and there was a significant positive correlation between them. 5. In AM group, the expression of TrkA increased with the increase of serum CA125 level, and there was a significant positive correlation between them. Conclusion: the expression of TrkA is significantly increased in adenomyosis, and is positively correlated with the degree of pain, suggesting that TrkA may be involved in the occurrence of dysmenorrhea in adenomyosis.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R711.71

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