CXCL10在子痫前期患者胎盘组织中的表达及意义

发布时间：2018-03-19 01:37

本文选题：CXCL10　切入点：子痫前期　出处：《山西医科大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的：通过研究正常孕晚期妇女和子痫前期重度患者胎盘组织中趋化因子CXCL10的表达情况，探讨CXCL10在子痫前期患者胎盘组织中的表达及意义。方法：选取30例正常孕晚期妇女和30例子痫前期患者作为对照组和研究组，采用原位杂交方法测定两组胎盘组织中CXCL10mRNA的表达水平，并且应用免疫组化方法检测两组胎盘组织中CXCL10的表达水平，通过医学图像分析系统对实验结果进行定量检测并作比较。数据资料应用SPSS17.0统计软件进行统计学处理，，两样本均数比较采用独立样本t检验。结果：（1）原位杂交方法显示CXCL10mRNA在正常孕晚期妇女和子痫前期患者胎盘组织的滋养细胞胞浆中有阳性表达，CXCL10mRNA在子痫前期患者胎盘组织中的表达（平均灰度值：144.52±3.46）明显高于其在正常孕晚期妇女胎盘组织中的表达（平均灰度值：156.04±2.78），差异有统计学意义（P＜0.05）；（2）免疫组化方法显示CXCL10在正常孕晚期妇女和子痫前期患者胎盘组织的滋养细胞胞浆中有阳性表达，CXCL10在子痫前期患者胎盘组织中的表达（平均灰度值：98.29±3.58）明显高于其在正常孕晚期妇女胎盘组织中的表达（平均灰度值：118.90±3.29），差异有统计学意义（P＜0.05）。结论：原位杂交及免疫组化方法均显示CXCL10在正常妊娠组和子痫前期患者胎盘组织滋养细胞胞浆中有表达。两种实验方法均提示CXCL10在子痫前期患者胎盘组织中的阳性表达高于正常妊娠组，提示CXCL10的升高与子痫前期的发病机制有关。
[Abstract]:Objective: to study the expression of chemokine CXCL10 in placenta of normal women with late pregnancy and severe preeclampsia, and to explore the expression and significance of CXCL10 in placenta of preeclampsia. Methods: 30 normal women with late pregnancy and 30 patients with preeclampsia were selected as control group and study group. In situ hybridization was used to detect the expression of CXCL10mRNA in placenta of the two groups. The expression of CXCL10 in two groups of placentas was detected by immunohistochemical method, and the experimental results were quantitatively detected and compared by the medical image analysis system. The data were processed statistically by SPSS17.0 statistical software. The mean of two samples was compared by independent sample t test. Results:. (1) in situ hybridization method showed that the expression of CXCL10 mRNA in placental cytoplasm of normal pregnant women and preeclampsia patients was significantly higher than that of CXCL10 mRNA in placenta of preeclampsia (mean gray value: 144.52 卤3.46). The expression in placenta of normal women in late pregnancy (mean gray value: 156.04 卤2.78) was significantly different (P < 0.05). (2) Immunohistochemical method showed that the expression of CXCL10 in placental cytoplasm of normal women with late pregnancy and preeclampsia was significantly higher than that of CXCL10 in placenta of preeclampsia (mean gray value: 98.29 卤3.58). The expression (mean gray value: 118.90 卤3.29) of placenta was significantly higher than that of normal women (P < 0.05) during the third trimester of pregnancy. Conclusion: the expression of CXCL10 in placental trophoblast cytoplasm of normal pregnancy group and preeclampsia group is demonstrated by in situ hybridization and immunohistochemistry. Both methods suggest that CXCL10 is expressed in placenta of preeclampsia. The positive expression was higher in normal pregnancy group than in normal pregnancy group. It is suggested that the increase of CXCL10 is related to the pathogenesis of preeclampsia.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R714.244

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