MiRNA-22在不同分期浆液性卵巢腺癌中的表达以及与化疗药物耐药性关系的研究

发布时间：2018-03-20 10:13

本文选题：卵巢肿瘤　切入点：腺癌　出处：《肿瘤学杂志》2016年12期 　论文类型：期刊论文

【摘要】：[目的]探讨miRNA-22在不同分期浆液性卵巢腺癌的表达以及与化疗耐药性关系。[方法 ]使用基因芯片技术筛选出差异化表达的miRNA,再用实时荧光定量聚合酶链反应(RT-PCR)检测miRNA-22在人卵巢癌细胞株(SKOV3)、紫杉醇稳定耐药卵巢浆液性囊腺癌细胞亚株(SKOV3-TR30)、人正常卵巢上皮细胞株(IOSE80)及50例确诊为浆液性卵巢腺癌的患者、25例正常人血清中的表达水平,并分析其与患者临床分期的关系以及用药前后的关系。[结果]miRNA-22在SKOV3-TR30耐药株中的表达明显高于SKOV3敏感株中的表达水平(P0.05),IOSE80正常卵巢上皮细胞株中miRNA-22的表达水平明显低于SKOV3敏感株(P0.05);在FIGO手术病理不同分期中的表达,Ⅰ、Ⅱ、Ⅲ、Ⅳ期表达水平依次增高;经统一的化疗方案治疗后,化疗无效组的表达水平高于化疗有效组的表达水平(P0.05)。[结论]miRNA-22可能是卵巢癌患者潜在的肿瘤标志物,miRNA-22的高表达可能与癌细胞的过度增殖与转移、侵袭有关,具有癌基因与抑癌基因的双侧作用,且可能与化疗药物的耐药性密切相关。
[Abstract]:[Objective] to investigate miRNA-22 using gene chip technology to screen differentiated expression in different stage of serous ovarian adenocarcinoma expression and chemotherapy resistance. The relationship between the miRNA method and real-time fluorescence quantitative polymerase chain reaction (RT-PCR) and miRNA-22 was detected in human ovarian cancer cell line (SKOV3), paclitaxel resistant ovarian serous cystadenocarcinoma cell line (SKOV3-TR30), normal ovarian epithelial cell line (IOSE80) and 50 cases of serous ovarian adenocarcinoma patients, the expression level of 25 cases of normal human serum, and analyze the relationship between the expression of its relationship with clinical staging before and after the treatment. The results of]miRNA-22 and SKOV3-TR30 in resistant strains significantly the expression level of SKOV3 was higher than that in the susceptible strain (P0.05), the expression level of IOSE80 in normal ovarian epithelial cells in miRNA-22 were significantly lower than that of sensitive strains of SKOV3 (P0.05) FIGO in different pathology; The expression in I, II, III, IV expression level increased after chemotherapy; unified treatment, expression of ineffective chemotherapy group was higher than that in chemotherapy effective group (P0.05). Conclusion]miRNA-22 may be a potential tumor marker of ovarian cancer patients, proliferation and metastasis, high expression of miRNA-22 may be related to cancer cell invasion, bilateral with the role of oncogenes and tumor suppressor genes, and drug resistance and chemotherapy are closely related.

【作者单位】：天门市第一人民医院;
【分类号】：R737.31

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