MiR-34a对JAR细胞自噬影响的机制

发布时间：2018-03-21 16:20

  本文选题：子痫前期　切入点：JAR细胞　出处：《山东医药》2017年09期 　论文类型：期刊论文

【摘要】：目的探讨miR-34a对人胎盘绒毛癌细胞JAR细胞自噬影响的机制。方法选取JAR细胞,随机分为miR-34a模拟物组(转染miR-34a模拟物)、NC组(转染模拟物阴性对照)、miR-34a抑制物组(转染miR-34a抑制物)、INC组(转染抑制物阴性对照)、空白对照组(只加Lipofectamine 2000)、缺氧组(300μmol/L氯化钴处理)和正常组(未经氯化钴处理)。采用Real time PCR法检测各组miR-34a及HMGB1 mRNA表达,Western blotting法检测各组HMGB1和LC3Ⅱ/Ⅰ蛋白表达,透射电镜检测各组细胞中自噬小体形成情况。结果 miR-34a模拟物组中HMGB1 mRNA和蛋白表达水平较NC组降低(P0.05),miR-34a抑制物组中HMGB1 mRNA和蛋白表达较INC组高(P0.05)。缺氧组中HMGB1和LC3Ⅱ/Ⅰ蛋白表达较正常组升高。缺氧处理后miR-34a模拟物组中HMGB1和LC3Ⅱ/Ⅰ蛋白的相对表达量较INC组降低(P0.05),miR-34a抑制物组中HMGB1和LC3Ⅱ/Ⅰ蛋白的相对表达量较INC组升高(P0.05)。透射电镜结果显示,缺氧组自噬小体的数量较正常组明显增加(P0.05),缺氧处理后miR-34a模拟物组细胞中自噬小泡数量较NC组减少(P0.05),miR-34a抑制物组细胞中自噬小泡数量较INC组增加(P0.05)。结论 miR-34a可能通过抑制HMGB1表达影响滋养细胞的自噬。
[Abstract]:Objective to investigate the effect of miR-34a on autophagy of human placental choriocarcinoma JAR cells. Methods JAR cells were selected. The miR-34a mimics were randomly divided into two groups: the miR-34a mimic group (NC-transfected miR-34a mimic) group (miR-34a negative control group) (transfection of miR-34a inhibitor + INC group), the blank control group (Lipofectamine 2000 alone, hypoxia group containing 300 渭 mol/L Cobalt chloride). Real time PCR method was used to detect the expression of miR-34a and HMGB1 mRNA in each group. Western blotting method was used to detect the expression of HMGB1 and LC3 鈪，

本文编号：1644616