血清和糖皮质激素诱导的蛋白激酶-1和缺氧在子痫前期发病作用的研究

发布时间：2018-03-22 12:22

本文选题：子痫前期　切入点：血清和糖皮质激素诱导的蛋白激酶-　出处：《重庆医科大学学报》2015年10期 　论文类型：期刊论文

【摘要】：目的:研究血清和糖皮质激素诱导的蛋白激酶-1(serum and glucocorticoids-inducible kinase-1,SGK1)在子痫前期胎盘组织中的分布和表达,以及缺氧对胎盘滋养细胞SGK1表达的影响。方法:选择2012年12月至2013年11月在重庆医科大学附属第二医院剖宫产分娩的50例子痫前期孕妇为研究对象,其中子痫前期轻度组25例,子痫前期重度组25例,以同期行择期性剖宫产术的25例正常孕妇为对照组。免疫组化检测SGK1在胎盘组织的分布及表达,Western blot检测SGK1在胎盘组织蛋白水平的表达。在常氧条件下(21%O2、5%CO2)和缺氧条件下(1%O2、5%CO2)培养HTR-8/SVneo滋养层细胞,Western blot检测SGK1蛋白的表达。结果:免疫组化结果表明子痫前期轻度组、重度组SGK1的蛋白表达量均较对照组明显增加(P=0.000),子痫前期重度组SGK1的蛋白表达量较子痫前期轻度组明显增加(P=0.028)。Western blot结果表明子痫前期轻度组、重度组SGK1的蛋白表达量均较对照组明显增加(P=0.000),子痫前期重度组SGK1的蛋白表达量较子痫前期轻度组明显增加(P=0.000)。Masson染色结果显示子痫前期轻度组、重度组的纤维化率均较对照组明显增加(P=0.000),子痫前期重度组纤维化率较子痫前期轻度组明显增加(P=0.000)。细胞实验部分:Western blot结果表明缺氧组SGK1的蛋白表达量较常氧组明显增加(P=0.000);缺氧组CTGF的蛋白表达量较常氧组明显增加(P=0.001)。Masson染色结果显示缺氧组纤维化率较常氧组明显增加(P=0.000)。结论:SGK1在子痫前期胎盘组织表达明显升高,提示SGK1可能在子痫前期的发病中发挥重要作用,缺氧通过刺激SGK1表达升高,参与子痫前期的发生。
[Abstract]:Objective: to study the distribution and expression of serum and glucocorticoid induced protein kinase (and glucocorticoids-inducible kinase-1) SGK1 in placenta of preeclampsia. Methods: from December 2012 to November 2013, 50 preeclampsia pregnant women who were delivered by cesarean section in the second affiliated Hospital of Chongqing Medical University were selected as the study subjects, including 25 cases of mild preeclampsia group. 25 cases of severe preeclampsia group, The distribution and expression of SGK1 in placenta tissue were detected by immunohistochemistry. The protein expression of SGK1 in placenta tissue was detected by Western blot. Under normoxic condition, there were 25 normal pregnant women undergoing elective cesarean section as control group. The expression of SGK1 protein was detected by Western blot in cultured HTR-8/SVneo trophoblastic cells. Results: immunohistochemical results showed that there were mild preeclampsia groups. The protein expression of SGK1 in severe group was significantly higher than that in control group. The protein expression of SGK1 in severe preeclampsia group was significantly higher than that in mild preeclampsia group. The results showed that the protein expression of SGK1 in severe preeclampsia group was significantly higher than that in mild preeclampsia group. The protein expression of SGK1 in severe group was significantly higher than that in control group. The protein expression of SGK1 in severe preeclampsia group was significantly higher than that in mild preeclampsia group. The results showed that the protein expression of SGK1 in severe preeclampsia group was significantly higher than that in mild preeclampsia group. The fibrosis rate in severe group was significantly higher than that in control group, and the fibrosis rate in severe preeclampsia group was significantly higher than that in mild preeclampsia group. The results of cell experiment showed that the protein expression of SGK1 in hypoxia group was higher than that in normal oxygen group. The expression of CTGF protein in hypoxia group was significantly higher than that in normal oxygen group. The results showed that the fibrosis rate of hypoxia group was significantly higher than that of normal oxygen group. Conclusion the expression of CTGF protein in placenta of preeclampsia was significantly higher than that of normal oxygen group. It is suggested that SGK1 may play an important role in the pathogenesis of preeclampsia. Hypoxia plays an important role in the pathogenesis of preeclampsia by stimulating the increase of SGK1 expression.
【作者单位】：重庆医科大学附属第二医院妇产科;
【分类号】：R714.244

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