糖原合成酶激酶3β对卵巢癌顺铂耐药性的影响及其机制研究

发布时间：2018-03-23 10:43

本文选题：糖原合成酶激酶β　切入点：卵巢癌　出处：《南京医科大学学报(自然科学版)》2015年03期

【摘要】：目的 :探讨糖原合成酶激酶3β(glycogen synthase kinase,GSK-3β)对卵巢癌顺铂耐药性的影响及机制。方法 :运用氯化锂(lithium chloride,Li Cl)增加失活性GSK-3β即p-GSK-3β表达,MTT法筛选合适的Li Cl浓度和作用时间并检测顺铂的半数抑制浓度,流式细胞仪检测p-GSK-3β(ser9)的表达量,免疫荧光观察其细胞内定位情况。结果:据MTT结果选择Li Cl 20 mmol/L处理6 h,处理后24、48、72 h,SKOV3细胞顺铂的半数抑制浓度从处理前的(20.37±0.56)、(7.79±0.72)、(2.8±0.32)μg/ml增加至(27.57±0.81)、(12.15±1.00)、(5.84±0.64)μg/ml,差异均有统计学意义。流式结果显示,Li Cl处理后SKOV3细胞内p-GSK-3β(ser9)的平均荧光强度由处理前的661.67±37.63增加到1 191.33±30.37,差异有统计学意义(P0.05)。免疫荧光结果提示,Li Cl处理后SKOV3细胞核内p-GSK-3β(ser9)表达上调。结论:Li Cl预处理后卵巢癌细胞顺铂耐药性的增强可能与GSK-3β失活性磷酸化水平增加,尤其是与核内水平上调有关。
[Abstract]:Objective: to investigate the effect and mechanism of glycogen synthase kinase 3 尾 -glycogen synthase kinase- GSK-3 尾 on cisplatin resistance in ovarian cancer. Methods: Lithium chloridede LiCl) was used to increase the inactivation of GSK-3 尾, or p-GSK-3 尾 expression. The half inhibitory concentration of cisplatin was detected. Flow cytometry was used to detect the expression of p-GSK-3 尾 -ser9. Results: the intracellular localization of SKOV3 cells was observed by immunofluorescence. Results: according to the MTT results, the median inhibitory concentration of cisplatin in SKOV3 cells was increased from 7.79 卤0.72 卤0.72 渭 g/ml to 27.57 卤0.81 卤1.00 卤5.84 渭 g / ml, respectively. The results showed that the average fluorescence intensity of SKOV3 cells increased from 661.67 卤37.63 to 1 191.33 卤30.37 after LiCl treatment, and the difference was statistically significant (P 0.05). The immunofluorescence results suggested that the expression of p-GSK-3 尾 ser9 in SKOV3 cells was up-regulated after LiCl treatment. The increase of cisplatin resistance may be associated with the increase of GSK-3 尾 deactivated phosphorylation. In particular, it is related to the upregulation of nuclear level.
【作者单位】：桂林医学院附属医院生殖中心;
【基金】：广西壮族自治区自然科学基金(0728235)
【分类号】：R737.31

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