上皮性卵巢癌中八聚体结合转录因子4、Notch1及DLL4的表达及其临床意义

发布时间：2018-03-23 13:14

本文选题：上皮性卵巢癌　切入点：八聚体结合转录因子　出处：《南方医科大学学报》2017年04期

【摘要】：目的探讨上皮性卵巢癌(EOC)中八聚体结合转录因子4(OCT4)、Notch1及DLL4蛋白表达之间的关系及其功能意义。方法收集207例EOC术后标本和65例卵巢良性上皮性肿瘤标本,应用免疫组化学法检测EOC和卵巢良性上皮性肿瘤组织中OCT4、Notch1以及DLL4蛋白的表达情况。结果 EOC组织和卵巢良性上皮性肿瘤组织中,OCT4、Notch1和DLL4蛋白的阳性表达率分别为60.0%、61.8%、60.9%和9.2%、6.2%、0,差异均有统计学意义(P0.05);OCT4、Notch1和DLL4的表达与EOC的组织学分化、FIGO分期、盆腔淋巴结转移有关(P0.05);DLL4蛋白的表达分别与OCT4和Notch1的表达呈正相关关系(r值分别为0.758和0.704,P均0.001),同时,OCT4与Notch1蛋白的表达亦呈正相关(r=0.645,P0.001)。Kaplan-Meier生存分析表明,OCT4、Notch1和DLL4蛋白的过表达均与患者的生存率有关,3种蛋白表达阳性组患者生存率均明显低于蛋白表达阴性组患者(P0.05)。多因素分析:OCT4、DLL4蛋白的表达和FIGO分期是影响EOC根治术后患者预后的独立因素(P0.05)。结论OCT4、Notch1及DLL4的过表达与EOC的组织学分化程度、转移和预后等因素密切相关,联合检测这些指标可能对于EOC患者的病情进展和预后判断给予重要提示。
[Abstract]:Objective to investigate the relationship between the expression of octamer binding transcription factor 4OCT4tch1 and DLL4 protein in epithelial ovarian carcinoma (EOC). Methods 207 cases of EOC postoperative specimens and 65 cases of ovarian benign epithelial tumors were collected. The expression of OCT4ntch1 and DLL4 protein in EOC and ovarian benign epithelial tumors were detected by immunohistochemical method. Results the positive rates of OCT4Notch1 and DLL4 in EOC and ovarian benign epithelial tumors were 60.0% and 60.9%, respectively. There were significant differences in the expression of OCT4Notch1 and DLL4 and the histological differentiation of EOC in Figo stage. The expression of DLL4 protein in pelvic lymph node metastasis was positively correlated with the expression of OCT4 and Notch1. The r values were 0.758 and 0.704, respectively. The expression of OCT4 and Notch1 protein was also positively correlated with the expression of Notch1 protein. The survival analysis showed that OCT4Notch1 and DLL4 protein were overexpressed. The survival rate of the patients with positive expression of three proteins was significantly lower than that of the patients with negative protein expression (P 0.05). Multivariate analysis showed that the expression of DLL4 protein and the FIGO stage were independent of the prognosis of patients after EOC radical operation. Conclusion the overexpression of OCT4, Notch1 and DLL4 and the histological differentiation of EOC may be related to the expression of OCT4, Notch1 and DLL4. The factors such as metastasis and prognosis are closely related. The combined detection of these indicators may be an important indication for the progression and prognosis of EOC patients.
【作者单位】：蚌埠医学院第一附属医院病理科//蚌埠医学院病理学教研室//安徽省感染与免疫重点实验室;蚌埠医学院临床医学系;
【基金】：安徽省高校自然科学研究重点项目(KJ2017A224) 安徽省高等学校省级优秀青年人才基金项目(2012SQRL094) 蚌埠医学院自然科学类重点项目(BYKY1416ZD) 国家级大学生创新训练项目(201610367015)
【分类号】：R737.31

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