1.5T MRI工作条件下暴露对人滋养细胞swan71的功能、结构及蛋白表达的影响

发布时间：2018-03-23 21:17

  本文选题：MRI　切入点：孕早期　出处：《浙江大学》2014年硕士论文

【摘要】：目的： 近年来,胎儿磁共振成像(Fetal magnetic resonance imaging, FMRI)在产科领域发展较快,其用于胎儿宫内成像相较于目前常用的超声具有无以比拟的优越性。但是妊娠时期MRI检查的安全性一直存在争议。目前没有证据表明临床使用MRI会对胎儿的发育、出生及成长产生不良的影响,但动物实验中一些研究却观察到了畸形的发生。所以尽管大多认为MRI无电离辐射,但其潜在的、尚不清楚的生物学效应对胎儿的影响目前还不确切。另一方面,孕前3个月由于胚胎处于细胞分化发育期,容易受外界各种物理因素的损伤,这一时期胚胎异常发生率很高。但妊娠早期MRI对胎儿的副作用至今无相关研究,MRI生殖毒性的特征及其机制尚不清楚。蛋白质组学是近年来发展起来的高通量筛查技术,主要在整体水平研究机体蛋白质的组成及其活动规律。它为从全局的角度,动态、整体研究胎儿MRI对孕早期胚胎命活动中复杂的蛋白质功能的影响提供了一种可能的高效手段,为胎儿MRI安全性探索提供了另一种全新的视角与途径。 本课题拟以孕早期人滋养细胞Swan71为研究对象,蛋白质组学为切入点,研究临床常用的1.5T胎儿MRI长时间工作的情况下的潜在影响。 方法： 1.将人滋养细胞Swan71细胞在1.5T MRI常规工作条件下暴露60min后,采用WST-1检测细胞增殖、流式细胞术检测细胞周期,评估MRI暴露对孕早期人滋养细胞Swan71细胞功能的影响。 2.将人滋养细胞Swan71细胞在1.5TMRI常规工作条件下暴露60min后,采用透射电镜技术观察观察细胞亚显微结构变化研究MRI对人滋养细胞Swan71的损伤作用； 3.将人滋养细胞Swan71细胞在1.5TMRI常规工作条件下暴露60min后,通过iTRAQ技术分析暴露组与对照组蛋白表达谱出现的差异,并初步分析这些蛋白质的功能作用。 结果： 1.经MRI暴露60分钟后,人滋养细胞swan71细胞与对照组相比,增殖抑制明显(p0.05),细胞周期无明显改变。 2.电镜观察：暴露后线粒体肿胀明显,体积变大变圆,基质着色变浅、嵴变短变少。 3.用iTRAQ定量蛋白组学方法比较分析经MRI暴露60分钟和对照组之间人滋养细胞swan71细胞蛋白质表达谱是否存在差异。最终发现79个差异蛋白,其中49个上调,30个下调,这些差异蛋白参与细胞生命及胚胎发育的重要方面。 结论： 1.60分钟MRI暴露对孕早期人滋养细胞株swan71的功能与结构可产生一定程度的影响。 2.MRI暴露可导致孕早期人滋养细胞株swan71蛋白质表达谱发生改变。 3. ITRAQ蛋白质谱分析是筛选MRI暴露相关差异蛋白表达的有效方法,为研究MRI对子代安全性研究提供新的视角。
[Abstract]:Objective:
In recent years, fetal magnetic resonance imaging (Fetal magnetic resonance imaging, FMRI) and rapid development in obstetrics, which is used for fetal imaging compared with the commonly used ultrasound has its incomparable superiority. But the safety inspection of the MRI period of pregnancy has been controversial. There is no evidence for clinical use of MRI for fetal development birth, and adverse effects on growth, but animal experiments in some studies have observed malformations. Although most of that MRI non ionizing radiation, but its potential is not clear, the biological effect of fetal impact is not exact. On the other hand, during the 3 months since the embryo in the cell the differentiation and development stage, various physical factors are susceptible to external damage, the embryo abnormal rate is very high. But the side effects of early pregnancy on fetal MRI has no related research, reproductive MRI The characteristics and mechanism of toxicity is unclear. Proteomics is a high-throughput screening technology developed in recent years, mainly in the study of the overall level of body protein composition and activities. It is from a global perspective, dynamic, overall impact of fetal MRI on protein function of complex life activities in early pregnancy embryo offers an efficient means possible, provides a new perspective and approach to explore the safety of fetal MRI.
The aim of this study is to take human gestational trophoblast Swan71 in early pregnancy as the research object. Proteomics is the breakthrough point to study the potential impact of 1.5T MRI on the long term work.
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