母鼠维生素D缺乏对子代鼠肺发育及CyclinD1表达影响的研究

发布时间：2018-03-24 07:33

本文选题：维生素D　切入点：肺发育　出处：《中国优生与遗传杂志》2017年03期

【摘要】：目的观察大鼠在孕期、哺乳期维生素D(Vitamin D)缺乏对子代鼠的肺组织结构发育及细胞周期蛋白D1(CyclinD1)表达的影响。方法成年雌性SD大鼠完全随机分配为维生素D缺乏模型组(VDD组)及正常对照组(对照组),每组共6只,缺D组予完全避光、不含维生素D饲料喂养,正常组予正常光照、正常饲料喂养,2周后,与成年雄性SD大鼠以2:1比例合笼交配,建立Vit D孕鼠缺乏模型。利用竞争性酶联免疫吸附试验技术(ELISA)检测母鼠产后21天血清25-(OH)-D3的水平。分别取出生后第1、7、14、21天子代鼠肺组织,光镜下分别观察肺组织形态结构,免疫组化法分别检测肺组织中CyclinD1的表达。结果 VDD组雌鼠血清25-(OH)-D3水平显著低于对照组(P0.05);光镜下同日龄VDD组肺组织结构较对照组肺泡间隔增宽,肺泡组织少,肺泡小而不规则,终末细支气管及小支气管形态不规则;免疫组化法检测CyclinD1 VDD组及对照组均在支气管黏膜上皮细胞表达明显,对照组CyclinD1在生后第1天表达最强,第7、14、21天逐渐减弱,而VDD组CyclinD1在生后第7天表达最强,高于对照组(P0.05),第1天表达低于对照组(P0.05),第14、21天逐渐减弱,均高于对照组,差异有统计学意义(P0.05)。结论母鼠孕期、哺乳期维生素D缺乏延缓子代鼠的肺发育过程,CyclinD1参与肺组织细胞的增殖,CyclinD1表达延迟可能是母鼠Vit D缺乏抑制子代鼠肺发育的可能机制之一。
[Abstract]:Objective to observe rats during pregnancy, During lactation, vitamin D Vitamin D) deficiency affected lung tissue development and cyclin D 1 expression in offspring rats. Methods Adult female SD rats were randomly assigned to vitamin D deficiency model group (VDD group) and normal control group. (control group, 6 rats in each group), Group D was fed without vitamin D, and group D was fed with normal light. After feeding with normal diet for 2 weeks, adult male Sprague-Dawley rats were mated in the cage at 2:1. A model of Vit D pregnant mice was established. The serum 25-(OH)-D3 levels were detected by competitive enzyme-linked immunosorbent assay (Elisa) on the 21st day postpartum. The lung tissue was taken from the first day of birth and the lung tissue was observed under light microscope. Results the level of serum 25-(OH)-D3 in the VDD group was significantly lower than that in the control group (P 0.05), and the lung tissue structure of the VDD group at the same day of age was wider than that of the control group under light microscope, and the alveolar tissue in the same age group was smaller and irregular than that in the control group. The morphology of terminal bronchioles and bronchioles was irregular, the expression of CyclinD1 in CyclinD1 VDD group and control group was obvious in bronchial epithelial cells, the expression of CyclinD1 in control group was the highest on the 1st day after birth, and gradually decreased on day 7 1421. On the 7th day after birth, the expression of CyclinD1 in the VDD group was the strongest, which was higher than that in the control group (P 0.05). The expression of CyclinD1 in the VDD group was lower than that in the control group on the first day, and gradually decreased on the 14th day after birth, which was higher than that in the control group, and the difference was statistically significant (P 0.05). The delayed expression of cyclin D1 in lung tissue cells may be one of the possible mechanisms by which Vit D deficiency in female mice inhibits the lung development of offspring.
【作者单位】：南昌大学第一附属医院儿科;
【基金】：江西省卫生计生委科技项目,项目编号:700009003
【分类号】：R714.2

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