miR-146a在上皮性卵巢癌中的表达及调控机制初步研究

发布时间：2018-03-25 01:28

本文选题：上皮性卵巢癌　切入点：miR-146a　出处：《中南大学》2014年硕士论文

【摘要】：目的：检测miR-146a在正常卵巢组织、良性上皮性卵巢肿瘤组织、上皮性卵巢癌组织中的表达水平并结合临床病理参数资料进行分析,初步探讨miR-146a在上皮性卵巢癌中发生发展过程中的可能作用；进一步检测正常卵巢组织和上皮性卵巢癌组织中miR-146a靶基因IR-AK1蛋白分子表达情况,初步探讨miR-146a在上皮性卵巢癌中可能的分子调控通路。研究方法： 1.采用实时荧光定量PCR法检测正常卵巢组织、良性上皮性卵巢肿瘤组织、上皮性卵巢癌组织中miR-146a的相对表达水平,并分析miR-146a表达和各临床病理参数的关系。 2.运用Western-Blotting检测正常卵巢组织和上皮性卵巢癌组织中miR-146a靶基因IRAK1蛋白分子相对表达水平并分析上皮性卵巢癌中miR-146a与IRAK1表达相关性。结果： 1.在正常的卵巢组织、良性上皮性卵巢肿瘤以及上皮性卵巢癌组织中,miR-146a的相对表达量逐渐升高,与正常卵巢组织相比,miR-146a表达在良性上皮性卵巢肿瘤及上皮性卵巢癌组织中明显升高,差异有统计学意义(P0.05)；且上皮性卵巢癌组织中miR-146a的表达明显高于良性上皮性卵巢肿瘤,差异有统计学意义(P0.05)。 2. miR-146a的相对表达量在低分化上皮性卵巢癌组中的明显高于高-中分化上皮性卵巢癌组,差异具有统计学意义(P=0.006)；在不同年龄、FIGO分期、CA125水平组中表达均无统计学意义(P均0.05)。 3.与正常卵巢组织相比,IRAKI蛋白表达水平在过表达miR-146a的上皮性卵巢癌组织中明显降低,差异具有统计学意义(P0.05),且两者表达水平呈明显的负相关(r=-0.78,P0.05)。结论： 1.上皮性卵巢癌组织中miR-146a表达水平相比于正常卵巢组织和良性卵巢上皮性肿瘤组织明显上调,可能参与了上皮性卵巢癌的发生及调控。 2.miR-146a表达水平在上皮性卵巢癌低分化组中明显高于高-中分化组,miR-146a可能与上皮性卵巢癌肿瘤细胞恶性程度有关。 3.过表达miR-146a的上皮性卵巢组织中IRAK1蛋白分子显著下调,两者呈显著负相关,在蛋白分子水平上支IRAK1是miR-146a的靶基因,miR-146a可能通过靶向抑制IRAK1mRNA的翻译,调控上皮性卵巢癌细胞的生物学功能。
[Abstract]:Objective:. The expression of miR-146a in normal ovarian tissue, benign epithelial ovarian tumor tissue and epithelial ovarian carcinoma tissue was detected and analyzed with clinicopathological data. To explore the possible role of miR-146a in the occurrence and development of epithelial ovarian cancer, and to detect the expression of miR-146a target gene IR-AK1 protein in normal ovarian tissues and epithelial ovarian cancer tissues. To explore the possible molecular regulatory pathway of miR-146a in epithelial ovarian cancer. Research methods:. 1. The relative expression of miR-146a in normal ovarian tissues, benign epithelial ovarian tumor tissues and epithelial ovarian cancer tissues was detected by real-time fluorescence quantitative PCR. The relationship between miR-146a expression and clinicopathological parameters was analyzed. 2. The relative expression of miR-146a target gene IRAK1 protein in normal ovarian tissues and epithelial ovarian carcinoma tissues was detected by Western-Blotting and the correlation between miR-146a and IRAK1 expression in epithelial ovarian carcinoma was analyzed. Results:. 1. In normal ovarian tissues, the relative expression of miR-146a in benign epithelial ovarian tumors and epithelial ovarian cancer tissues increased gradually. The expression of miR-146a in benign epithelial ovarian tumor and epithelial ovarian carcinoma was significantly higher than that in normal ovarian tissue (P 0.05), and the expression of miR-146a in epithelial ovarian carcinoma was significantly higher than that in benign epithelial ovarian tumor. The difference was statistically significant (P 0.05). 2. The relative expression of miR-146a in poorly differentiated epithelial ovarian carcinoma group was significantly higher than that in well-moderately differentiated epithelial ovarian carcinoma group, and the difference was statistically significant (P < 0.05). 3. The expression of IRAKI protein in epithelial ovarian carcinoma with overexpression of miR-146a was significantly lower than that in normal ovarian tissues, and the difference was statistically significant (P 0.05), and there was a significant negative correlation between the expression level of IRAKI and the expression of IRAKI protein in epithelial ovarian carcinoma tissues with overexpression of miR-146a. Conclusion:. 1. The expression of miR-146a in epithelial ovarian carcinoma was significantly higher than that in normal ovarian tissues and benign ovarian epithelial tumor tissues, which may be involved in the genesis and regulation of epithelial ovarian cancer. The expression of 2.miR-146a in the poorly differentiated epithelial ovarian carcinoma group was significantly higher than that in the high and medium differentiation group. The expression of miR-146a might be related to the malignancy of epithelial ovarian cancer cells. 3. IRAK1 protein molecules were significantly down-regulated in epithelial ovarian tissues with overexpression of miR-146a, and there was a significant negative correlation between them. At the protein molecular level, IRAK1 was the target gene of miR-146a, and miR-146a might inhibit the translation of IRAK1mRNA by targeting. The biological function of epithelial ovarian cancer cells is regulated.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.31

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