TSLP和NF-κB在子宫腺肌病子宫内膜细胞中的表达及意义

发布时间：2018-03-25 17:20

本文选题：胸腺基质淋巴细胞生成素（TSLP）　切入点：胸腺基质淋巴细胞生成素受体（TSLPR）　出处：《山西医科大学》2014年硕士论文

【摘要】：目的研究TSLP、TSLPR和NF-κB三种蛋白在子宫腺肌病患者子宫内膜腺上皮细胞和间质细胞中的表达及血清TSLP水平，探讨TSLP、TSLPR和NF-κB在子宫腺肌病发病机制中的作用。方法采用免疫组织化学SABC法检测35例子宫腺肌病患者异位、在位内膜及20例子宫肌瘤患者子宫内膜腺上皮细胞和间质细胞中TSLP、TSLPR和NF-κBp65的表达，并分别对TSLP和NF-κBp65，TSLP和TSLPR两组蛋白表达量进行相关性分析；采用ELISA双抗体夹心法检测患者血清TSLP的水平，子宫肌瘤患者和健康体检者各20例作为对照。采用Image-proPlus5.1图像分析系统对图像进行分析，用SPSS17.0统计软件进行数据处理。结果子宫腺肌病患者异位、在位子宫内膜腺上皮细胞和间质细胞中TSLP、TSLPR和NF-κBp65三种蛋白均阳性表达，且表达量明显高于对照组（P0.01），异位内膜腺上皮细胞和间质细胞中TSLP、TSLPR和NF-κBp65表达量均高于在位内膜相应蛋白的表达量（P0.01），异位内膜腺上皮细胞和间质细胞中TSLP和NF-κBp65的表达呈正相关，而子宫腺肌病患者异位、在位子宫内膜腺上皮细胞和间质细胞中的TSLP和TSLPR的表达均无明显的相关性，，子宫腺肌病患者血清中TSLP的水平高于子宫肌瘤患者（P0.05）和健康体检者（P0.05）。结论（1）子宫腺肌病患者，在位内膜TSLP、TSLPR和NF-κB的表达高于对照组，表明子宫腺肌病患者在位内膜里有不同于正常内膜的特质，这也体现和证明了“在位内膜决定论”的观点；（2）子宫腺肌病患者异位内膜TSLP、TSLPR和NF-κB表达显著高于在位内膜的表达，这提示子宫腺肌病患者异位灶的形成可能与TSLP、TSLPR和NF-κB过表达有关。TSLP与TSLPR的作用可能通过延迟子宫内膜细胞的凋亡或通过自分泌作用促进异位子宫内膜细胞的增殖，造成异位灶的增殖和侵袭粘附作用；TSLP与TSLPR的作用通过作用于靶细胞产生炎症细胞因子，间接地促进Th2型细胞免疫从而导致局部炎症反应的发生，促进异位病灶的发展；（3）子宫腺肌病患者异位内膜腺上皮细胞和间质细胞中TSLP和NF-κB的表达呈正相关，异位内膜TSLP的高表达有可能与NF-κB的激活和过表达相关。
[Abstract]:Objective to investigate the expression of TSLP- TSLPR and NF- 魏 B in endometrial glandular epithelial cells and stromal cells and the level of serum TSLP in patients with adenomyosis, and to explore the role of TSLP- TSLPR and NF- 魏 B in the pathogenesis of adenomyosis. Methods the expression of TSLP- TSLPR and NF- 魏 Bp65 in endometrial epithelial cells and stromal cells of 35 patients with adenomyosis, eutopic endometrium and 20 patients with uterine leiomyoma were detected by immunohistochemical SABC method. The expression of TSLP and NF- 魏 Bp65 TSLP and TSLPR were analyzed respectively, and the serum TSLP levels were detected by ELISA double antibody sandwich method. 20 patients with uterine leiomyoma and 20 healthy controls were analyzed by Image-proPlus5.1 image analysis system, and the data were processed by SPSS17.0 software. Results TSLP- TSLPR and NF- 魏 Bp65 were positive in ectopic, eutopic endometrial glandular epithelial cells and stromal cells in patients with adenomyosis. The expression of TSLPnTSLPR and NF- 魏 Bp65 in ectopic endometrial glandular epithelial cells and stromal cells were significantly higher than those in eutopic endometrium. The expression of TSLP and NF- 魏 Bp65 in ectopic endometrial epithelial cells and stromal cells were positively correlated. However, there was no significant correlation between the expression of TSLP and TSLPR in ectopic, eutopic endometrial epithelial cells and stromal cells in patients with adenomyosis. The level of TSLP in serum of patients with adenomyosis was higher than that of patients with uterine leiomyosis (P0.05) and that of healthy controls. Conclusion (1) the expression of TSLP TSLPR and NF- 魏 B in eutopic endometrium in patients with adenomyosis is higher than that in control group, indicating that the eutopic endometrium of adenomyosis is different from normal endometrium. The expression of TSLP TSLPR and NF- 魏 B in ectopic endometrium was significantly higher than that in eutopic endometrium. These results suggest that the formation of ectopic foci in patients with adenomyosis may be related to the overexpression of TSLP TSLPR and NF- 魏 B. TSLP and TSLPR may promote the proliferation of ectopic endometrial cells by delaying the apoptosis of endometrial cells or by autocrine. The effects of TSLP and TSLPR on the proliferation, invasion and adhesion of ectopic foci can indirectly promote the Th2 type cell immunity and induce local inflammatory response by acting on the target cells to produce inflammatory cytokines. The expression of TSLP and NF- 魏 B in ectopic endometrial glandular epithelial cells and stromal cells was positively correlated with the development of ectopic lesions. The overexpression of TSLP in ectopic endometrium may be related to the activation and overexpression of NF- 魏 B.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R711.71

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