HPV16 E6及E7蛋白在宫颈癌组织中的表达与病理特征的相关性研究

发布时间：2018-03-25 17:05

  本文选题：宫颈癌　切入点：宫颈上皮内瘤变　出处：《中华医院感染学杂志》2016年17期

【摘要】：目的探讨人乳头瘤病毒(HPV)16E6及E7蛋白在宫颈癌前病变中宫颈癌组织的表达与病理特征的相关性,分析引起宫颈癌病变中的作用机制。方法收集2014年1月-2015年10月在医院手术的宫颈癌及宫颈上皮内瘤变(CIN)患者各60例,采用免疫组化Envision二步法对病变标本组织中HPV16E6、E7蛋白表达进行检测。结果宫颈癌组织中E6、E7表达阳性率分别为90.00%、88.33%,明显高于CIN组的68.33%、71.67%,差异有统计学意义(P0.05);鳞癌、分期Ⅲ-Ⅳ期患者宫颈癌组织中E6、E7表达阳性率分别为97.62%、97.62%、100.00%、83.87%,明显高于腺癌、分期Ⅰ-Ⅱ期的72.22%、66.67%、79.31%、58.62%,差异有统计学意义(P0.05),E6、E7表达阳性率与癌细胞分化程度无关;Ⅲ期患者CIN组织中E6、E7表达阳性率分别为86.96%、86.96%,明显高于Ⅰ-Ⅱ级的56.76%、62.16%,差异有统计学意义(P0.05)。结论 HPV16E6及E7蛋白在宫颈癌组织中阳性表达率较宫颈癌前病变明显增高,并与病理特征关系密切,检测HPV16E6及E7蛋白有可能作为宫颈癌前病变与宫颈癌转归的指标。
[Abstract]:Objective to investigate the correlation between the expression of HPV-16E6 and E7 proteins and the pathological features of cervical carcinoma in precancerous lesions of human papillomavirus. Methods from January 2014 to October 2015, 60 patients with cervical cancer and 60 patients with cervical intraepithelial neoplasia (cin) who were operated in hospital from January 2014 to October 2015 were analyzed. The expression of HPV16 E6P7 protein was detected by immunohistochemical Envision two-step method. Results the positive rates of E6 E7 expression in cervical carcinoma were 90.0088.33, which was significantly higher than that in CIN group (68.33%), the difference was statistically significant (P 0.05), and that in squamous cell carcinoma was significantly higher than that in CIN group (P < 0.05), and the expression of E7 protein in cervical carcinoma was significantly higher than that in CIN group (P < 0.05). The positive rate of E6N E7 expression in cervical cancer tissues of stage 鈪，

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