核黄素代谢途径影响顺铂对卵巢癌黏附迁移作用研究

发布时间：2018-03-25 16:16

  本文选题：核黄素　切入点：氯丙嗪　出处：《中国药学杂志》2017年17期

【摘要】：目的体外研究核黄素(RIB)代谢途径对顺铂(DDP)对卵巢癌HO8910细胞株黏附和迁移的作用。方法通过慢病毒包装核黄素转运体2(RFT2)shRNA干扰载体和RIB竞争性抑制剂氯丙嗪(CHL)干预RIB代谢途径。HO8910卵巢癌细胞随机分为空白对照组、shRNA对照组、RFT2 shRNA组、CHL(50μmol·L~(-1))组、DDP(20μmol·L~(-1))组、RFT2 shRNA+DDP组、CHL+DDP组和DDP+RIB(20μmol·L~(-1))组。各组细胞处理48 h后,细胞黏附实验检测细胞黏附能力;Transwell方法检测细胞迁徙能力;Western blot检测VEGF、MMP9和MMP2的表达;激光共聚焦检测TNF-α和NF-κB/p65的表达。结果与单独给药组对比,RFT2 shRNA或CHL联合DDP能够明显降低HO8910细胞黏附和迁移能力;降低MMP9和MMP2的表达;减少细胞TNF-α、NF-κB/p65的表达,RIB可以减弱DDP的作用。结论干扰FIB代谢途径能够增强DDP对卵巢癌HO8910细胞黏附和迁移能力的作用,该作用与抑制TNF-α/NF-κB途径有关。
[Abstract]:Objective to study the effect of riboflavin on the adhesion and migration of ovarian cancer HO8910 cell line by cisplatin DDP in vitro. Methods RIB was interfered with by lentivirus packaging riboflavin transporter 2(RFT2)shRNA interference vector and RIB competitive inhibitor chlorpromazine. Metabolic pathway. HO8910 ovarian cancer cells were randomly divided into two groups: blank control group, control group, RFT2 shRNA group, 50 渭 mol shRNA group) group, 20 渭 mol shRNA shRNA DDP group, chl DDP group and DDP RIB(20 渭 mol Lf-1 group, the cells in each group were treated for 48 h. Cell adhesion assay and Transwell method Western blot was used to detect the expression of VEGF MMP9 and MMP2. The expression of TNF- 伪 and NF- 魏 B/p65 was detected by confocal laser. Results compared with the control group, RFT2 shRNA or CHL combined with DDP could significantly reduce the adhesion and migration ability of HO8910 cells, decrease the expression of MMP9 and MMP2, and decrease the expression of MMP9 and MMP2. Conclusion interfering with the metabolic pathway of FIB can enhance the ability of DDP to adhesion and migration of ovarian cancer HO8910 cells, which is related to the inhibition of TNF- 伪 / NF- 魏 B pathway.
【作者单位】： 丽水学院医学与健康学院;丽水市妇幼保健院产科;
【基金】：浙江省医药卫生科学研究基金资助项目(2013KYA236) 丽水学院博士科研启动基金资助(QD1229)
【分类号】：R737.31
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本文编号：1663912