卵巢子宫内膜异位症异位病灶组织来源与其相关性不孕的关系

发布时间：2018-03-26 15:53

本文选题：卵巢子宫内膜异位症　切入点：不孕症　出处：《青岛大学》2017年硕士论文

【摘要】：目的:观察两个标记基因含黄素单氧化酶3(FMO3,在输卵管上皮组织高表达)、脑恶性肿瘤缺失基因(DMBT1,在子宫内膜组织高表达)分别在卵巢子宫内膜异位症(EMs)不孕患者和已育患者异位病灶组织及正常卵巢组织中m RNA和蛋白表达水平,探讨卵巢子宫内膜异位症的异位病灶组织不同来源是否与其相关性不孕有关。方法:收集2014年4月~2015年8月期间在青岛大学附属医院住院手术治疗患者,90例卵巢子宫内膜异位症患者根据是否伴有不孕分为卵巢EMs+不孕(40例)、卵巢EMs+已育(50例),两组异位病灶组织分别作为观察组和对照组。取因子宫肌瘤手术的35例患者正常卵巢组织作为正常组。采用Real-Time PCR法检测FMO3、DMBT1m RNA表达水平,采用Western blotting法检测FMO3、DMBT1蛋白表达水平。结果:1.观察组中两个标记基因FMO3、DMBT1在m RNA上表达水平均高于正常组,DMBT1上调为正常组的22.073-53.097(平均为33.953)倍,FMO3上调为正常组的2.058-6.096(平均为4.793)倍,且差异均有显著的统计学意义(P均0.05);2.对照组中仅有DMBT1在m RNA表达水平高于正常组,上调水平为正常组的5.892-12.436(平均为9.619)倍,两者间的差异有显著的统计学意义(P0.05)。FMO3在m RNA表达水平无明显增加,表达为正常组的1.501-2.131(平均为1.816)倍,两者间无显著的统计学意义(P0.05);3.观察组与对照组相比,观察组FMO3在m RNA表达水平明显升高,上调为1.738-8.500(平均为3.847)倍,差异有显著的统计学意义(P0.05)。DMBT1在m RNA的上调倍数为1.013-4.514(平均2.116),但其两组间的差异无显著的统计学意义(P0.05);4.与正常组DMBT1、FMO3的蛋白表达水平(分别为0.726-0.843(平均为0.780)、0.659-0.723(平均为0.699))相比,观察组中DMBT1、FMO3的蛋白表达水平均明显升高,分别为1.834-2.756(平均为1.600)、1.143-1.356(平均为1.259),且差异均具有显著的统计学意义;5.与正常组相比,对照组中DMBT1的蛋白表达水平明显升高,其表达水平为1.565-2.286(平均为1.400),差异有明显的统计学意义(P0.05)。FMO3蛋白表达水平为0.826-0.941(平均为0.880),两组中的表达水平无显著差异(P0.05);6.实验组与对照组相比,FMO3的蛋白表达水平明显升高,且差异具有显著的统计学意义(P0.05)。DMBT1的蛋白表达水平在两者间无明显区别,其差异不具有显著的统计学意义(P0.05)。结论:卵巢EMs相关性不孕患者的异位病灶组织高表达FMO3、DMBT1,提示不孕患者的异位病灶组织来源可能为输卵管上皮组织和子宫内膜组织;卵巢EMs合并已育患者异位病灶组织仅高表达DMBT1,FMO3的表达无明显升高,提示已育患者异位病灶组织可能来源为子宫内膜组织;输卵管组织来源的异位内膜组织可能与卵巢Ems患者不孕有关。
[Abstract]:Objective: to observe the expression of two marker genes, FMO3, in epithelial tissue of fallopian tube, and in ovarian endometriosis (EMs), respectively, and the deletion gene of DMBT1 in malignant tumor of brain (high expression in endometrial tissue); The expression level of m RNA and protein in ectopic focus tissue and normal ovarian tissue were detected. To investigate whether different sources of ectopic focus tissue in endometriosis of ovary are related to its associated infertility. Methods: from April 2014 to August 2015, 90 patients with ovarian cancer were treated by surgical treatment in Qingdao University affiliated Hospital. According to whether endometriosis is accompanied by infertility, 40 cases of ovarian EMs infertility and 50 cases of ovarian EMs have been divided into two groups: observation group and control group. 35 patients with uterine leiomyoma were treated as normal. Real-Time PCR assay was used to detect the expression of DMBT1m RNA in ovarian tissues. Western blotting method was used to detect the expression of FMO3 / DMBT1 protein. Results: 1. The expression level of two marker genes FMO3 / DMBT1 on m RNA in the observation group was higher than that in the normal group (22.073-53.097 (mean 33.953)) and the expression of FMO3 was 2.058-6.096 (mean 4.793) times higher than that in the normal group. The expression of DMBT1 in the control group was higher than that in the normal group, and the upregulation level was 5.892-12.436 (9.619) times higher than that in the normal group. There was no significant difference between the two groups in the expression level of m RNA, which was 1.501-2.131 times of normal group (average 1.816), and no significant statistical significance was found between the two groups. Compared with the control group, the expression of P0.05FMO3 in the observation group was higher than that in the control group, and there was no significant difference between the two groups. In the observation group, the expression of FMO3 in m RNA was significantly increased by 1.738-8.500 (mean 3.847) times. There was significant difference in the upregulation multiple of P0.05N. DMBT1 in m RNA (mean 2.116), but there was no significant difference between the two groups. The protein expression level of DMBT1 / FMO3 was 0.726-0.843 (mean 0.7800.659-0.723) in the normal group, but there was no significant difference between the two groups. The protein expression level of DMBT1 / FMO3 was significantly increased in the observation group (1.834-2.756 (mean 1.600-1.356), and the difference was significant statistically significant (P < 0.05). Compared with the control group, the expression of DMBT1 protein in the control group was significantly higher than that in the control group. The expression level of FMO3 was 1.565-2.286 (average 1.400), and the difference was significant (P 0.05) .FMO3 protein expression was 0.826-0.941 (mean 0.880). There was no significant difference between the two groups (P 0.05). Compared with the control group, the expression level of FMO3 in the experimental group was significantly higher than that in the control group, and the expression level of FMO3 in the experimental group was significantly higher than that in the control group, and the expression of FMO3 in the experimental group was significantly higher than that in the control group. There was no significant difference in the protein expression of P0.05U. DMBT1 between the two groups. Conclusion: the expression of FMO3 and DMBT1 in the ectopic lesions of patients with ovarian EMs associated infertility is high, suggesting that the ectopic lesions of infertile patients may originate from the tubal epithelial tissue and endometrial tissue. The expression of DMBT1FMO3 was not significantly increased in ovarian EMs complicated with ectopic lesions, suggesting that the ectopic focus tissues of fertile patients may originate from endometrial tissue. Ectopic endometrial tissue derived from oviduct tissue may be associated with infertility in patients with ovarian Ems.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R711.71;R711.6

【参考文献】