低分子柑橘果胶对子宫内膜癌细胞株Ishikawa中Caspase-3蛋白表达影响

发布时间：2018-03-28 03:06

本文选题：低分子柑橘果胶　切入点：子宫内膜癌　出处：《山东医药》2017年35期

【摘要】：目的观察低分子柑橘果胶(LMCP)对子宫内膜癌细胞株Ishikawa中半胱氨酸门冬氨酸蛋白酶-3(Caspase-3)表达的影响。方法 (1)将Ishikawa细胞随机分为A、B、C、D组,分别加入终浓度为0、100、300、500nmol/L的LMCP培养24 h,用Western blotting法检测各组Caspase-3。(2)将Ishikawa细胞随机分为1、2、3、4组,加入终浓度为300 nmol/L的LMCP分别培养0、6、12、36 h,用Western blotting法检测各组Caspase-3。结果 A、B、C、D组Ishikawa细胞中Caspase-3蛋白表达量分别为0.30±0.01、0.32±0.01、0.77±0.01、0.79±0.01。Caspase-3蛋白表达量A组B组C组D组,P均0.05。1、2、3、4组Ishikawa细胞中Caspase-3蛋白表达量分别为0.29±0.06、0.38±0.06、0.40±0.06、0.42±0.06。Caspase-3蛋白表达量1组2组3组4组,P均0.05。结论LMCP能增强子宫内膜癌细胞Ishikawa中凋亡蛋白Caspase-3表达,且此效果有剂量(0~500 nmol/L)和时间(0~36 h)依赖性。
[Abstract]:Objective to observe the effect of low molecular weight citrus pectin (LMC) on the expression of cysteine aspartate protease 3 (Caspase-3) in endometrial carcinoma cell line Ishikawa. The cells of Ishikawa were randomly divided into 4 groups (group 1, 2 and 3) after 24 h culture of LMCP with 0100300500nmol/L concentration. Caspase-3. 2 was detected by Western blotting assay. Caspase-3 was detected by Western blotting method. Results the expression of Caspase-3 protein in Ishikawa cells in group A was 0.30 卤0.01 卤0.77 卤0.01 卤0.79 卤0.01.Caspase-3 respectively. The expression of Caspase-3 protein in Ishikawa cells of group A, group B, group C, group C, group C, group C, group C, group C, group C, group C, group C, group A, group A, group A, respectively, the expression of Caspase-3 protein in Ishikawa cells of group A, group B, group B, group C, group C, P. The expression of 0.06.Caspase-3 protein was 0.29 卤0.06 + 0.38 卤0.06 + 0.40 卤0.06 + 0.42 卤0.06.Caspase-3 in group 1, group 2, group 3, group 3, group 4, P 0.05. Conclusion LMCP can enhance the expression of apoptotic protein Caspase-3 in Ishikawa of endometrial carcinoma cells. The effect was dependent on dose of 500 nmol / L and time of 36 h.
【作者单位】：中国医科大学第四附属医院;
【基金】：辽宁省自然科学基金项目(2015020482)
【分类号】：R737.33

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