胎盘生长因子、基质金属蛋白酶-2在子痫前期患者胎盘中的表达和意义

发布时间：2018-03-29 08:08

本文选题：子痫前期　切入点：基质金属蛋白酶-2　出处：《泰山医学院》2014年硕士论文

【摘要】：目的检测子痫前期（preeclampsia, PE）患者胎盘组织中胎盘生长因子（placenta growthfactors，PLGF）、基质金属蛋白酶-2（Matrix metalloproteinases-2, MMP-2）的表达，探讨其与子痫前期发病的关系。研究方法收集2012年8月~2013年10月在泰山医学院附属医院行常规产前检查并在该院剖宫分娩的子痫前期患者40例，，其中轻度子痫前期17例，重度子痫前期23例（子痫前期的诊断及分类标准参照乐杰主编的《妇产科学》第7版）。取同期正常晚妊选择性剖宫分娩孕妇15例作为对照组。采用免疫组织化学法检测轻度子痫前期组、重度子痫前期组及正常晚妊组胎盘组织中的胎盘生长因子、基质金属酶-2的表达。结果 1、正常胎盘组织中PLGF与MMP-2的细胞定位主要位于绒毛小叶的合体滋养细胞、细胞滋养细胞。阳性着色部位均位于胞浆。 2、子痫前期组胎盘组织中PLGF与MMP-2的细胞定位与正常胎盘组织中的细胞定位相同。阳性着色部位均位于胞浆，与正常胎盘分布一致，着色强度较正常明显减弱。 3、MMP-2在正常组、轻度子痫前期组、重度子痫前期组胎盘组织中的表达阳性率为93.3%、70.6%、39.1%；轻度子痫前期组和正常组比较差异无显著性（P0.05）；重度子痫前期组与正常组相比较差异有显著性（P0.05），轻度子痫前期组与重度子痫前期组比较差异有显著性（P0.05）。 4、PLGF在正常组、轻度子痫前期组、重度子痫前期组胎盘组织中表达的阳性率为53.3%、41.2%、13.0%；轻度子痫前期组和正常组比较差异无显著性（P0.05）；重度子痫前期组与正常组相比较差异有显著性（P0.05），轻度子痫前期组与重度子痫前期组比较差异有显著性（P0.05）。 5、重度子痫前期组中PLGF和MMP-2的表达水平存在着正相关性（r=0.697，P0.05）。结论 1、在正常组和子痫前期组的胎盘组织中，PLGF与MMP-2均有表达，其表达主要位于绒毛小叶的合体滋养细胞、细胞滋养细胞。 2、MMP-2和PLGF在轻、重度子痫前期组中的表达明显低于正常组胎盘组织。随着子痫前期病情的加重，胎盘组织中MMP-2和PLGF表达急剧减少。MMP-2和PLGF的表达异常可能是导致子痫前期发生的原因之一。 3、在重度子痫前期组中，MMP-2和PLGF的表达呈现正相关性。MMP-2和PLGF可能在滋养细胞的侵袭和胎盘血管重铸过程中相互影响，两者可能从多个途径引发子痫前期。
[Abstract]:Purpose. To investigate the relationship between placental growth factor (placenta growth factor) and matrix metalloproteinases-2 (MMP-2) in placental tissue of preeclampsia (PE) patients, the expression of placental growth factor (placenta) and matrix metalloproteinases-2 (MMP-2) in patients with preeclampsia were detected. Research method. From August 2012 to October 2013, 40 preeclampsia patients, 17 of whom had mild preeclampsia, underwent routine antenatal examination in the affiliated Hospital of Taishan Medical College and delivered in the hospital. 23 cases of severe preeclampsia (the diagnostic and classification criteria of preeclampsia refer to the 7th edition of Gynecology, edited by Ledger). 15 pregnant women with normal late pregnancy selective cesarean section were selected as the control group. Immunohistochemistry was used. Method to detect mild preeclampsia group, Expression of placental growth factor and matrix metallozyme -2 in placenta of severe preeclampsia group and normal late pregnancy group. Results. 1. The cellular localization of PLGF and MMP-2 in normal placenta was mainly located in syncytiotrophoblast and cytotrophoblast in villi lobule. 2. The cellular localization of PLGF and MMP-2 in placenta of preeclampsia was the same as that in normal placenta. 3MMP-2 in normal group, mild preeclampsia group, The positive rate of placental tissue expression in severe preeclampsia group was 93.30.60.61%, there was no significant difference between mild preeclampsia group and normal group (P 0.05), there was significant difference between severe preeclampsia group and normal group (P 0.05), and between mild preeclampsia group and severe preeclampsia group, there was a significant difference between severe preeclampsia group and severe preeclampsia group. There was significant difference in preeclampsia group (P 0.05). 4 PLGF in normal group, mild preeclampsia group, The positive rate of placental expression in severe preeclampsia group was 41.20.There was no significant difference between mild preeclampsia group and normal group (P 0.05); there was significant difference between severe preeclampsia group and normal group (P 0.05), and there was significant difference between mild preeclampsia group and heavy preeclampsia group (P 0.05). There was significant difference in preeclampsia group (P 0.05). 5, the expression of PLGF and MMP-2 in severe preeclampsia group was positively correlated with 0.697P0.05P in severe preeclampsia group. Conclusion. 1. The expression of PLGF and MMP-2 in placenta of normal group and preeclampsia group was mainly located in syncytiotrophoblast and cytotrophoblast in villi lobule. 2the expression of MMP-2 and PLGF in mild and severe preeclampsia group was significantly lower than that in normal placenta tissue. The abnormal expression of MMP-2 and PLGF may be one of the causes of preeclampsia. 3. In severe preeclampsia group, the expression of MMP-2 and PLGF was positively correlated. MMP-2 and PLGF may interact with each other in the process of trophoblast invasion and placental vascular recasting, and they may lead to preeclampsia in several ways.
【学位授予单位】：泰山医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R714.244

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