不同宫颈组织中SIRT-1蛋白的表达及其临床意义

发布时间：2018-03-30 07:29

本文选题：SIRT-1　切入点：western-blot　出处：《青岛大学》2017年硕士论文

【摘要】：目的:探讨正常子宫颈组织、宫颈不同级别上皮内瘤变组织(低级别上皮内瘤变组织、高级别上皮内瘤变组织)及宫颈癌组织中SIRT-1蛋白的表达及其临床意义。方法:收集2014-10-01至2016-01-30于青岛大学医学院附属医院妇科及青岛市妇女儿童医院妇科中心行手术治疗或宫颈活检确诊的患者140例,其中低级别上皮内瘤变组织(LSIL)26例,高级别上皮内瘤变组织(HSIL)35例及宫颈癌组织55例(包括47例鳞状细胞癌,8例非鳞癌)。另选择同期我院因子宫肌瘤行全子宫切除术后病理确诊为正常宫颈的患者24例为对照。应用免疫组织化学法及western-blot实验方法检测SIRT-1蛋白的表达情况,并探讨SIRT-1蛋白与宫颈癌患者临床及各病理特征之间的相关性,统计学分析采用SPSS19.0。结果:1、免疫组化实验结果显示SIRT-1蛋白主要在细胞核中表达,棕黄色颗粒状染色为阳性(见图3-1);在正常子宫颈组织、低级别上皮内瘤变组织、高级别上皮内瘤变组织、癌性宫颈组织中的阳性表达率分别为0、15.38%、37.14%、40.00%,差异有统计学意义(X2=16.82,P0.05)。2.western-blot实验方法检测中SIRT-1蛋白在正常宫颈组织、低级别宫颈上皮内瘤变组(LSIL)、高级别宫颈上皮内瘤变组(HSIL)及宫颈癌组中的表达情况与免疫组化实验的结果中SIRT-1蛋白表达基本上一致,四组间进行比较存在显著性差异,有统计学意义(F=13.86,P0.05)(见图3-2)。3.宫颈癌组织中SIRT-1蛋白的表达与病理分化程度、有无淋巴结转移及FIGO临床分期有关,分化程度越低阳性表达率越高,有显著性差异(X2=6.87,P0.05);淋巴结转移阳性组的阳性表达率高于淋巴结转移阴性组,差异有显著统计学意义(X2=7.73,P0.05);SIRT-1蛋白的阳性表达率随临床分期的增高而增加,有显著统计学意义(X2=7.39,P0.05)。4.SIRT-1蛋白的表达与宫颈癌的组织学类型、患者的年龄无关,差异无统计学意义(X2=1.03、0.038,P0.05)。结论:1.SIRT-1蛋白在正常宫颈组织、低级别上皮内瘤变组织(LSIL)、高级别上皮内瘤变组织(HSIL)、宫颈癌组织中的阳性表达率渐进性增高,提示SIRT-1可能参与了正常宫颈组织-低级别上皮内病变-高级别上皮内病变-宫颈癌发生发展的渐进过程。2.SIRT-1蛋白在组织病理学分化程度低、淋巴结转移阳性、临床分期晚的癌症患者中高表达,提示SIRT-1蛋白表达的异常可能在宫颈癌的发生、进展及转移的过程中起到了重要作用。3.SIRT-1蛋白有望成为宫颈癌的早期诊断及预后判断的生物学指标,为制定宫颈癌防治策略提供新的靶标和科学证据。
[Abstract]:Objective: to study the normal cervical tissue, cervical intraepithelial neoplasia tissue of different grades (low grade intraepithelial neoplasia tissue), The expression and clinical significance of SIRT-1 protein in high grade intraepithelial neoplasia) and cervical carcinoma. Methods: from 2014-10-01 to 2016-01-30, the patients were operated at the gynecology center of the affiliated Hospital of Qingdao University Medical College and the Gynecological Center of Qingdao Women and Children's Hospital. 140 patients diagnosed by treatment or cervical biopsy, Among them, 26 cases of low grade intraepithelial neoplasia were found. 35 cases of high grade intraepithelial neoplasia and 55 cases of cervical carcinoma (including 47 cases of squamous cell carcinoma and 8 cases of non-squamous cell carcinoma) were selected. 24 cases of normal cervix were pathologically diagnosed after hysterectomy in our hospital during the same period. Immunohistochemical method and western-blot assay were used to detect the expression of SIRT-1 protein. To explore the correlation between SIRT-1 protein and clinical and pathological features of cervical cancer patients, SPSS 19.0. results showed that SIRT-1 protein was mainly expressed in the nucleus. Brown-yellow granular staining was positive (see Fig. 3-1; in normal cervical tissues, low-grade intraepithelial neoplasia, high-grade intraepithelial neoplasia, The positive expression rates of SIRT-1 protein in cervical cancer tissues were 0.15.38 and 37.14, respectively. The difference was statistically significant (P 0.05). 2. Western blot was used to detect SIRT-1 protein in normal cervix. The expression of SIRT-1 protein in low grade cervical intraepithelial neoplasia group, high grade cervical intraepithelial neoplasia group and cervical carcinoma group was basically consistent with the results of immunohistochemistry, and there was significant difference among the four groups. The expression of SIRT-1 protein was related to the degree of pathological differentiation, lymph node metastasis and clinical stage of FIGO. The lower the differentiation degree, the higher the positive expression rate. The positive expression rate of SIRT-1 protein in positive lymph node metastasis group was higher than that in negative lymph node metastasis group, and the positive expression rate of SIRT-1 protein increased with the increase of clinical stage. There was significant statistical significance between the expression of X2O7.39 P0.05N. 4. The expression of SIRT-1 protein was not related to the histological type of cervical cancer, but there was no significant difference in age of the patients. Conclusion: 1. SIRT-1 protein is expressed in normal cervical tissues. The positive expression rate of LSILA in low grade intraepithelial neoplasia and HSIL in high grade intraepithelial neoplasia was increased gradually in cervical carcinoma. These results suggest that SIRT-1 may be involved in the progressive process of normal cervix, low grade intraepithelial lesion, high grade intraepithelial lesion and cervical carcinogenesis. 2. SIRT-1 protein has low histopathological differentiation and positive lymph node metastasis. The overexpression of SIRT-1 protein in patients with late clinical stage of cancer suggests that the abnormal expression of SIRT-1 protein may occur in cervical cancer. SIRT-1 protein plays an important role in the progression and metastasis of cervical cancer. 3. SIRT-1 protein is expected to be a biological index for early diagnosis and prognosis of cervical cancer and provide new target and scientific evidence for the development of cervical cancer prevention and treatment strategy.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.33

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