功能蛋白在氯化镧逆转卵巢癌顺铂耐药中的差异表达分析

发布时间：2018-03-30 18:26

本文选题：卵巢癌　切入点：顺铂耐药　出处：《广东医学》2017年13期

【摘要】：目的从蛋白质水平研究氯化镧对人卵巢癌顺铂耐药细胞(COC1/DDP)功能蛋白表达的影响,为探讨氯化镧逆转卵巢癌顺铂耐药的机制提供实验依据。方法取对数生长期COC1/DDP细胞分为4组,对照组只加入10%胎牛血清培养基培养,顺铂组加入23.08μg/m L顺铂,氯化镧组和氯化镧+顺铂组分别加入1.5μmol/L氯化镧,处理8 h后氯化镧+顺铂组加入23.08μg/m L顺铂。采用蛋白印迹(Western blot)技术检测顺铂和氯化镧分别作用于COC1/DDP后,4组中ERCC1、Ki67、CDK6、HDAC2、c-Cbl和微管末端结合蛋白1(EB1)6种功能蛋白的表达变化。结果与对照组相比,COC1/DDP的4种功能蛋白ERCC1、Ki67、CDK6、c-Cbl在顺铂组和顺铂+氯化镧组中表达显著下调,差异有统计学意义(P0.05);且顺铂+氯化镧组较顺铂组表达明显下调,差异有统计学意义(P0.05)。与对照组比较,顺铂组中HDAC2和EB1表达明显下调,差异有统计学意义(P0.05);顺铂+氯化镧组与顺铂组、氯化镧组中表达比较有下降趋势,但差异无统计学意义(P0.05)。结论氯化镧可能通过多种途径下调ERCC1、Ki67、CDK6和c-Cbl表达,从而介导逆转卵巢癌顺铂耐药。
[Abstract]:Objective to study the effect of lanthanum chloride on the expression of functional protein of cisplatin resistant human ovarian cancer cell line (COC1 / DDP) at protein level, and to provide experimental evidence for the mechanism of lanthanum chloride reversing cisplatin resistance in ovarian cancer. Methods COC1/DDP cells at logarithmic growth stage were divided into 4 groups. The control group was cultured in 10% fetal bovine serum medium, the cisplatin group was added 23.08 渭 g / mL cisplatin, the lanthanum chloride group and lanthanum cisplatin group were added 1.5 渭 mol/L lanthanum chloride, respectively. After 8 hours of treatment, lanthanum cisplatin group was added 23.08 渭 g / mL cisplatin. Western blotting technique was used to detect the expression of ERCC1 / Ki67CDK6HDAC2Cbl and microtubule terminal binding protein (1(EB1)6) functional proteins in ERCC1 / Ki67CDK6HDAC2Cbl and microtubule terminal binding protein (1(EB1)6) groups respectively. Compared with the control group, the four functional proteins of COC1 / DDP, ERCC1 / Ki67, CDK6Cbl, were significantly down-regulated in cisplatin and lanthanum chloride groups. The expression of HDAC2 and EB1 in cisplatin lanthanum chloride group was significantly lower than that in cisplatin group, and the difference was statistically significant. Compared with the control group, the expression of HDAC2 and EB1 in cisplatin group was significantly down-regulated. The expression of CDK6 and c-Cbl in lanthanum chloride group was lower than that in cisplatin group and lanthanum chloride group, but the difference was not statistically significant. Conclusion the expression of CDK6 and c-Cbl may be down-regulated by lanthanum chloride. Thus mediated reversal of cisplatin resistance in ovarian cancer.
【作者单位】：南昌大学第一附属医院妇产科;
【基金】：国家自然科学基金资助项目(编号:81260381)
【分类号】：R737.31

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