MUC4、MUC16及HER2与卵巢癌癌变机制及临床应用关系的研究

发布时间：2018-03-30 19:45

本文选题：卵巢癌　切入点：细胞系　出处：《扬州大学》2014年硕士论文

【摘要】：目的：检测MUC4、MUC16及HER2在卵巢癌细胞系中的表达情况,探索其与卵巢癌发生发展的关系,及其在卵巢癌早期诊断、治疗和预后监测中的作用和意义。方法：对人卵巢癌SKOV-3细胞株、OVCAR-3细胞株、CAOV-3细胞株进行细胞培养；应用RT-PCR技术检测人卵巢癌三种细胞中MUC4、MUC16及HER2mRNA水平的表达情况；采用细胞免疫荧光法及Westen Blot法检测人卵巢癌三种细胞中MUC4、MUC16及HER2蛋白水平的表达情况。结果：(1)RT-PCR检测结果显示：MUC4mRNA在人卵巢癌CAOV-3细胞中表达量最高,在OVCAR-3细胞中次之,在SKOV-3细胞中最低,差异无统计学意义(P0.05); MUC16mRNA在人卵巢癌OVCAR-3细胞中表达量最高,在CAOV-3细胞中次之,在SKOV-3细胞中表达量最低,差异具有统计学意义(P0.05); HER2mRNA在人卵巢癌CAOV-3细胞中表达量最高,在OVCAR-3细胞中次之,在SKOV-3细胞中表达量最低,在SKOV-3细胞和OVCAR-3细胞中表达量差异无统计学意义(P0.05),而在SKOV-3细胞和CAOV-3细胞、OVCAR-3细胞和CAOV-3细胞中差异具有统计学意义(P0.05)。 (2)细胞免疫荧光法和Westen Blot法检测发现：MUC4蛋白在人卵巢癌SKOV-3细胞和OVCAR-3细胞中不表达,在CAOV-3细胞中有表达；MUC16蛋白在人卵巢癌SKOV-3细胞、OVCAR-3细胞、CAOV-3细胞中均有表达,并且在OVCAR-3细胞中表达最高,在CAOV-3细胞次之,在SKOV-3细胞中表达最低,差异具有统计学意义(P0.05); HER2蛋白在SKOV-3细胞、OVCAR-3细胞和CAOV-3细胞中均有表达,且在CAOV-3细胞中表达最高,在OVCAR-3细胞次之,在SKOV-3细胞中表达最低,差异具有统计学意义(P0.05)。结论：(1)MUC4及、MUC16及HER2与卵巢癌的发生发展有密切的关系,并可能成为卵巢癌治疗的新靶点。 (2)CA125(MUC16)在早期和晚期卵巢癌中表达水平不同可能与其在不同类型卵巢癌中不表达或低表达有关。 (3)MUC4、MUC16及HER2在不同卵巢癌细胞系中表达水平不一,MUC4可能单独或联合CA125(MUC16)作为卵巢癌临床诊断、病情监测的辅助实验室指标。
[Abstract]:Objective: to detect the expression of MUC4, MUC16 and HER2 in ovarian cancer cell lines, and explore their relationship with the occurrence and development of ovarian cancer, and its role and significance in early diagnosis, treatment and prognosis monitoring of ovarian cancer.
Methods: the human ovarian cancer SKOV-3 cell line, OVCAR-3 cell line, CAOV-3 cell line was cultured; three MUC4 cells in human ovarian cancer detection using RT-PCR technology, the expression of MUC16 and HER2mRNA levels; using immunofluorescence method and Westen Blot method to detect three kinds of MUC4 cells in human ovarian carcinoma, the expression of MUC16 the HER2 and protein levels.
Results: (1) RT-PCR test results showed that: MUC4mRNA in human ovarian cancer cell CAOV-3 expression was the highest in OVCAR-3 cells in the second, the lowest in SKOV-3 cells, there was no statistically significant difference (P0.05); MUC16mRNA in human ovarian cancer cell OVCAR-3 expression was the highest in CAOV-3 cells of SKOV-3 expression was the lowest, the difference was statistically significant (P0.05); HER2mRNA in human ovarian cancer cell CAOV-3 expression was the highest in OVCAR-3 cells of the lowest expression in SKOV-3 cells, SKOV-3 cells and OVCAR-3 cells in the expression of the difference was not statistically significant (P0.05), and in SKOV-3 cells and CAOV-3 cells, the difference was statistically significant in OVCAR-3 cells and CAOV-3 cells (P0.05).
(2) to detect cell immunofluorescence method and Westen Blot method: the expression of MUC4 protein in human ovarian cancer SKOV-3 cells and OVCAR-3 cells, are expressed in CAOV-3 cells; MUC16 protein OVCAR-3 in human ovarian cancer SKOV-3 cells, cells were expressed in CAOV-3 cells, and the highest expression in the OVCAR-3 cells in CAOV-3 cells, in SKOV-3 cells was the lowest, the difference was statistically significant (P0.05); HER2 protein in SKOV-3 cells expressed in OVCAR-3 cells and CAOV-3 cells, and expressed in CAOV-3 cells, OVCAR-3 cells in the SKOV-3 cells was the lowest, the difference was statistically significant (P0.05).
Conclusion: (1) MUC4 and MUC16 and HER2 are closely related to the development and development of ovarian cancer, and may be a new target for the treatment of ovarian cancer.
(2) the difference in expression level of CA125 (MUC16) in early and late ovarian cancer may be associated with non expression or low expression in different types of ovarian cancer.
(3) MUC4, MUC16 and HER2 have different expression levels in different ovarian cancer cell lines. MUC4 may be alone or combined with CA125 (MUC16) as an auxiliary laboratory index for ovarian cancer clinical diagnosis and disease surveillance.

【学位授予单位】：扬州大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.31

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