TLR4信号介导内膜间质细胞侵袭增殖促子宫腺肌症发病的研究

发布时间：2018-03-31 17:57

本文选题：子宫腺肌症　切入点：间质细胞　出处：《现代妇产科进展》2015年09期

【摘要】：目的:探讨Toll样受体4(TLR4)信号活化内膜间质在腺肌症发生与发展中的调控作用。方法:Western blot法检测腺肌病在位子宫内膜及病灶内膜中TLR4表达情况;建立原代间质体系,lipopolysaccharide(LPS)刺激靶细胞,CCK-8技术观察细胞增殖活力;Western blot法检测TLR4核心信号表达;Western blot法和酶联免疫吸附测定(ELISA)技术检测和分析间质细胞炎性增殖及侵袭生长相关因子表达;采用TLR4拮抗剂VIPER处理细胞,验证TLR4信号对间质生物效应的影响。结果:TLR4表达于细胞质膜,在腺肌症病灶内膜中表达最高;LPS刺激使间质细胞活力增强、TLR4核心信号及增殖侵袭因子表达增加;VIPER可抑制上述因子表达。结论:LPS/TLR4信号活促进内膜间质细胞炎性增殖、侵袭生长,在子宫腺肌症发病中发挥重要作用,提示宫腔感染可能与腺肌病发病具有一定的相关性。
[Abstract]:Aim: to investigate the role of Toll like receptor 4 (TLR 4) signal activation in the pathogenesis and development of adenomyosis.Methods the expression of TLR4 in eutopic endometrium and endometrium of adenomyosis was detected by Western blot.To establish a primary interstitial system of lipopolysaccharide (LPS) -stimulated target cells to observe cell proliferation activity; Western blot assay was used to detect the expression of TLR4 core signal; Elisa technique was used to detect and analyze the expression of inflammatory proliferation and invasive growth related factors in interstitial cells.Cells were treated with TLR4 antagonist VIPER to verify the effect of TLR4 signal on interstitial biological effects.Results the expression of TLR4 was expressed on the cytoplasmic membrane. The highest expression of lipopolysaccharide (LPS) in the intima of adenomyosis could enhance the activity of interstitial cells and increase the expression of TLR4 core signal and proliferative invasion factor. VIPER could inhibit the expression of these factors.Conclusion the activity of TLR4 signal activates the inflammatory proliferation, invasion and growth of endometrial stromal cells and plays an important role in the pathogenesis of adenomyosis, suggesting that intrauterine infection may be related to the pathogenesis of adenomyosis.
【作者单位】：同济大学附属杨浦医院妇产科;同济大学医学院妇科微创医学研究所;
【基金】：国家自然科学基金(No:81172461)
【分类号】：R711.71

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