卵母细胞经冷冻步骤改良的玻璃化法冻融后临床效果分析

发布时间：2018-04-01 21:24

本文选题：卵母细胞　切入点：玻璃化冷冻　出处：《广东医学》2017年S2期

【摘要】：目的探讨卵母细胞经冷冻步骤改良的玻璃化法冻融后行卵胞浆内单精子显微注射(ICSI)受精的临床效果。方法回顾性对比分析经改良法冻融后的卵母细胞(解冻卵组)与同期基本资料与其匹配的ICSI患者的新鲜卵母细胞(新鲜卵组)受精后的临床结局。结果解冻卵组和新鲜卵组的受精率(77.6%vs 76.1%)、卵裂率(98.5%vs 100.0%)、D3优质胚胎率(36.2%vs 27.5%)、优质囊胚(47.7%vs 47.3%)及可利用囊胚比率(52.2%vs 52.6%)差异无统计学意义;解冻卵组有62.5%的患者子宫内膜准备是自然周期,新鲜卵组所有患者均是超促排卵周期;但解冻卵的临床妊娠率及继续妊娠率分别为87.5%和81.3%,均显著性高于新鲜卵组的59.2%和51.3%;流产率新鲜卵组略高(7.1%vs 13.3%),但差异无统计学意义。结论改良后的冻卵方法极大地改善了卵母细胞的发育潜能,几乎无异于新鲜卵母细胞。
[Abstract]:Objective to investigate the clinical effect of vitrification and thawing oocytes by freezing and thawing. Methods the oocytes (thawed oocytes) after freezing and thawing (thawed oocyte group) were analyzed retrospectively. The clinical outcome of fertilized fresh oocytes (fresh oocytes) of ICSI patients matched with basic data of the same period. Results the fertilization rate of thawed egg group and fresh egg group was 77.6 vs 76.1g, the cleavage rate was 98.5 vs 100.00.The rate of good quality embryo was 36.2 vs 27.5B. There was no significant difference in the ratio of available blastocysts (52.2 vs 52.6B). In thawed egg group, 62.5% of the patients had a natural period of endometrial preparation, and all the patients in the fresh egg group had hyperstimulation cycle. However, the clinical pregnancy rate and continuous pregnancy rate of thawed eggs were 87.5% and 81.3%, respectively, which were significantly higher than those of fresh eggs group (59.2% and 51.3%), and the abortion rate of fresh eggs group was slightly higher than that of fresh eggs group, but the difference was not statistically significant. Improved the developmental potential of oocytes, Almost like fresh oocytes.
【作者单位】：广州医科大学附属第三医院生殖助孕科;
【基金】：国家自然科学基金资助项目(编号:81502507) 广东省教育厅基金资助项目(编号:2014KTSCX101) 广东省科技计划项目(编号:2013B021800273) 广州市荔湾区科信局基金资助项目(编号:20141217058) 广州市科技计划项目(编号:201607010200) 广州医科大学博士启动项目(编号:2015C31)
【分类号】：R714.8

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