两种不同胚胎培养模式对体外胚胎发育和妊娠结局的影响

发布时间：2018-04-02 06:35

本文选题：培养模式　切入点：胚胎质量　出处：《生殖与避孕》2015年01期

【摘要】：目的:比较2种胚胎培养模式对胚胎质量和妊娠结局的影响。方法:收集首次行IVF-ET治疗、年龄38岁、采用长方案促排卵、常规IVF授精、正常受精卵数3枚且第3日有新鲜胚胎移植的518个周期的临床资料,根据正常受精卵采用的培养模式分成单胚胎培养组(n=254)和胚胎群组培养组(n=264)。分析患者的基本资料、胚胎发育情况以及妊娠结局。结果:患者的年龄、不孕年限、基础卵泡刺激素(FSH)、Gn用量、h CG注射日内膜厚度以及各种不孕因素的比例等参数组间差异均无统计学意义(P0.05);平均获卵数、成熟率、2PN率等指标组间也均无统计学差异(P0.05),但胚胎群组培养组的卵裂率、第3日优质胚胎率、剩余胚胎可利用囊胚形成率均明显高于单胚胎培养组,差异有统计学意义(P0.05);胚胎群组培养组的种植率和临床妊娠率高于单胚胎培养组,但差异无统计学意义(P0.05)。结论:采用胚胎群组培养模式可以得到更多的优质胚胎和更高的囊胚形成率,推荐可用于日常IVF治疗周期中的胚胎培养。
[Abstract]:Objective: to compare the effects of two kinds of embryo culture models on embryo quality and pregnancy outcome. Methods: the first IVF-ET therapy, aged 38 years, was used to promote ovulation with long regimen and conventional IVF insemination. There were 3 normal fertilized eggs and 518 cycles of fresh embryo transfer on the 3rd day. According to the culture model of normal fertilized eggs, they were divided into single embryo culture group and embryo group culture group. The basic data of the patients were analyzed. Embryonic development and pregnancy outcome. Results: age of the patient, years of infertility, There was no significant difference in endometrial thickness and the proportion of various infertility factors between the two groups, and there was no significant difference between the two groups, and the average number of eggs obtained was higher than that of the control group. There was no significant difference in maturation rate and 2PN rate between the two groups, but the cleavage rate, the third high quality embryo rate and the blastocyst formation rate in the group of embryo group culture were significantly higher than those in the group of single embryo culture, and the rate of blastocyst formation was significantly higher in the group of embryo group culture than in the group of single embryo culture. The implantation rate and clinical pregnancy rate of embryo group culture group were higher than that of single embryo culture group. Conclusion: more high quality embryos and higher blastocyst rate can be obtained by using the embryo group culture model, and it is recommended that it can be used in the embryo culture of daily IVF treatment cycle.
【作者单位】：温州医科大学附属第二医院生殖中心;
【基金】：国家自然科学基金项目(81370674) 温州市科技局项目(Y20100205)
【分类号】：R714.8

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