先兆流产与MTHFR、MTRR基因多态性的关系

发布时间：2018-04-02 06:40

本文选题：叶酸代谢障碍　切入点：流产　出处：《重庆医学》2017年06期

【摘要】：目的调查叶酸代谢相关基因5,10-亚甲基四氢叶酸还原酶(MTHFR)、甲硫氨酸合成酶还原酶(MTRR)多态性与先兆流产的关系。方法选择海南省三亚市妇幼保健院在2009-2011的139例具有先兆流产症状的育龄妇女(病例组)和139例健康对照(对照组)来进行病例对照研究,通过刮取受检者口腔黏膜上皮细胞,提取其基因组DNA,对受检者MTHFR和MTRR基因多态性采用荧光定量PCR进行分析检测。通过分析病例组和对照组基因多态性的分布差异,评估其与先兆流产的相关性。结果病例组和对照组基因分布均符合Hardy-Weinberg平衡定律,但经比较分析,病例组MTHFR C677T、MTHFR A1298C和MTRR A66G的基因型构成与对照组比较差异均无统计学意义(P0.05)。结论在先兆流产患者和健康人中,基因多态性分布无显著差异,叶酸代谢相关基因是否为先兆流产的风险因子还有待进一步研究。
[Abstract]:Objective to investigate the relationship between the polymorphisms of 5-methylenetetrahydrofolate reductase (MTHFR), methionine synthase reductase (MTRR) and threatened abortion.Methods A case-control study was conducted in 139 women (case group) and 139 healthy controls (control group) with threatened abortion symptoms in Sanya Maternal and Child Health Hospital of Hainan Province in 2009-2011. Oral mucosal epithelial cells were obtained by scraping.The genomic DNA was extracted and the polymorphism of MTHFR and MTRR genes was analyzed by fluorescence quantitative PCR.To evaluate the relationship between gene polymorphism and threatened abortion by analyzing the distribution of gene polymorphisms between case group and control group.Results the gene distribution in both the case group and the control group was in accordance with the law of Hardy-Weinberg equilibrium. However, the genotype composition of MTHFR C677T MTHFR A1298C and MTRR A66G in the case group was not significantly different from that in the control group (P 0.05).Conclusion there is no significant difference in the distribution of gene polymorphisms between threatened abortion patients and healthy persons. Whether folic acid metabolism related genes are risk factors of threatened abortion remains to be further studied.
【作者单位】：重庆市黔江区中心医院妇产科;海南省三亚市妇幼保健院妇产科;上海靶向分子医学研究所;海南主健细胞分子遗传医学检验中心;中国疾病预防控制中心妇幼保健中心;
【基金】：中国疾病预防控制中心妇幼保健中心妇幼保健分子遗传医学研究专项计划(FY-ZX-ZD-0050) 三亚市医疗卫生科技创新项目(YW1231)
【分类号】：R714.21

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