孕妇显性和隐匿性乙肝感染分子进化特征

发布时间：2018-04-04 22:38

  本文选题：隐匿性乙型肝炎病毒感染　切入点：显性乙型肝炎病毒感染　出处：《中国公共卫生》2016年11期

【摘要】：目的探讨孕妇人群显性和隐匿性乙型肝炎病毒(HBV)感染及其HBV病毒株S基因的分子进化特征。方法 2014年6月1日—31日,在知情同意下,连续招募安徽省安庆市立医院产科初次住院活胎孕妇156人,收集其人口学特征和血清;对所有血清平行进行乙型肝炎病毒表面抗原(HBsAg)酶联免疫吸附法和电化学发光法检测、应用实时PCR定量测定HBV DNA、应用巢式PCR进行HBV S片段扩增、测序及进化分析。结果156名孕妇中,显性和隐匿性HBV感染率分别为10.3%(16/156)、8.6%(12/140);成功获得13株HBV S基因序列,其中显性7株,隐匿性6株;所有病毒株均为B基因型;仅1例隐匿性HBV株为ayw1血清型,其余12株均为adw2血清型。结论孕妇人群携带较高比例的显性和隐匿性HBV。
[Abstract]:Objective to investigate the dominant and occult hepatitis B virus (HBV) infection in pregnant women and the molecular evolution of S gene of HBV virus strain.Methods from June 1 to 31, 2014, 156 pregnant women were recruited from Anhui Anqing Municipal Hospital for the first time in obstetrics department with informed consent to collect their demographic characteristics and serum.All sera were detected by enzyme linked immunosorbent assay (Elisa) and electrochemiluminescence assay (ECL) for hepatitis B virus surface antigen HBsAg (HBsAg), real-time PCR was used to quantify HBV, nested PCR was used to amplify HBV S fragment, sequencing and evolution analysis were performed.Results among 156 pregnant women, the dominant and occult HBV infection rates were 10.3 / 156 / 8.66 / 140, respectively, and 13 HBV S gene sequences were successfully obtained, of which 7 were dominant and 6 were occult. All the virus strains were B genotypes, and only one occult HBV strain was ayw1 serotype.The other 12 strains were all adw2 serotypes.Conclusion pregnant women carry a high proportion of dominant and occult HBV.
【作者单位】： 复旦大学公共卫生学院卫生微生物学教研室;复旦大学公共卫生学院教育部公共卫生重点实验室;复旦大学卫生部卫生技术评估重点实验室;安徽省安庆市立医院检验科;安徽省安庆市立医院妇产科;复旦大学公共卫生学院卫生统计学教研室;
【基金】：国家自然科学基金(81373065) 上海市教育委员会科研创新重点项目(14ZZ015)
【分类号】：R714.251
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