PCOS患者卵泡液微环境中的亚急性炎症反应

发布时间：2018-04-06 03:27

本文选题：多囊卵巢综合征　切入点：肥胖　出处：《中国病理生理杂志》2015年09期

【摘要】：目的:回顾性分析多囊卵巢综合征(PCOS)患者及对照者卵泡液微环境中局部脂代谢紊乱及炎症反应状态及其形成机制。方法:比较2组患者外周血清脂代谢指标、性激素、炎症细胞及炎症介质水平差异;测定卵泡液中炎症介质及载脂蛋白含量;分析卵巢颗粒细胞中炎症介质及载脂蛋白基因表达。结果:PCOS患者具备典型肥胖、高脂血症及高雄激素血症,外周血中炎症细胞及各类炎症介质水平显著增加;卵泡液中同样具有显著增加的炎症细胞因子浸润,颗粒细胞内炎症介质及载脂蛋白基因表达增加。结论:PCOS组患者卵泡液内所含高水平载脂蛋白表明卵泡液内可能存在高脂蛋白状态。循环中的高脂血症及亚急性炎症各类因子可能通过血清-卵泡液屏障主动渗透。颗粒细胞通过主动摄取(例如载脂蛋白A)抑或自主合成(如炎症因子IL-1、IL-6及TNF-α等)等多种方式进行卵泡液局部微环境的稳态调节。卵子发育生理性卵泡液微环境的脂质代谢紊乱、炎症细胞浸润、高雄激素血症等均可能影响到卵母细胞和胚胎的正常发育。
[Abstract]:Objective: to retrospectively analyze the local lipid metabolism disorder and inflammatory reaction in follicular fluid microenvironment of patients with polycystic ovary syndrome (PCOS) and control group.Methods: the levels of serum lipid metabolism, sex hormones, inflammatory cells and inflammatory mediators were compared between the two groups, and the levels of inflammatory mediators and apolipoproteins in follicular fluid were measured.Inflammatory mediators and apolipoprotein gene expression in granulosa cells were analyzed.Results patients with PCOS had typical obesity, hyperlipidemia and hyperandrogenemia. The levels of inflammatory cells and various inflammatory mediators in peripheral blood were significantly increased, and the infiltration of inflammatory cytokines in follicular fluid was also significantly increased.Inflammatory mediators and apolipoprotein gene expression increased in granulosa cells.Conclusion the high level of apolipoprotein in follicular fluid may indicate the existence of high lipoprotein in follicular fluid.Circulating hyperlipidemia and subacute inflammatory factors may permeate through the serum-follicular fluid barrier.Granulosa cells regulate the local microenvironment of follicular fluid by active uptake (such as apolipoprotein A) or spontaneous synthesis (such as inflammatory cytokines IL-1- 6 and TNF- 伪).The disorder of lipid metabolism, infiltration of inflammatory cells and hyperandrogenemia may affect the normal development of oocytes and embryos.
【作者单位】：中山大学附属第六医院生殖医学中心;
【基金】：国家自然科学基金资助项目(No.81100401;No.81470063) 广东省自然科学基金资助项目(No.2014A030313129)
【分类号】：R711.75

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