骨桥蛋白在宫腔粘连患者子宫内膜中的表达及意义

发布时间：2018-04-08 10:07

本文选题：宫腔粘连　切入点：骨桥蛋白　出处：《中南大学》2014年硕士论文

【摘要】：目的：探讨骨桥蛋白(osteopontin,OPN)在宫腔粘连患者子宫内膜中的表达情况及其在宫腔粘连发病机制中的作用。方法：采用免疫组织化学法(immunohistochemistry,IHC)及逆转录-聚合酶链反应(reverse transcription-PCR,RT-PCR)法,分别检测70例宫腔粘连(intrauterine adhension, IUA)患者子宫内膜及30例子宫纵隔患者正常子宫内膜组织中OPN的蛋白及mRNA的表达情况,比较宫腔粘连组与正常组子宫内膜OPN的表达量的差异,以及不同程度宫腔粘连组子宫内膜OPN的表达量的差异,并对结果进行分析。结果：1.免疫组织化学检测结果：OPN蛋白主要在子宫内膜腺体表达,间质有少量表达。IUA组的表达高于非IUA组,差异具有统计学意义(P0.05)；OPN蛋白在重度IUA组的表达高于中度IUA组,中度IUA组的表达高于轻度IUA组,差异具有统计学意义(P0.05)。2.逆转录-聚合酶链反应法检测结果：OPNmRNA在IUA组的表达高于非IUA组,差异具有统计学意义(P0.05)；重度IUA组的表达高于中度IUA组,中度IUA组的表达高于轻度IUA组,差异具有统计学意义(P0.05),与免疫组织化学检测结果相符。结论：1.OPN在宫腔粘连患者子宫内膜中表达明显增强,提示OPN可能与宫腔粘连的发生有关；2.随着宫腔粘连程度的加重,OPN的表达逐渐增强,提示OPN可能与宫腔粘连的严重程度相关。
[Abstract]:Objective: to investigate the expression of osteopontinin (OPN) in endometrium of patients with intrauterine adhesion and its role in pathogenesis of intrauterine adhesion.Methods: the expression of OPN protein and mRNA in endometrium of 70 cases of intrauterine adhesion (IUAA) and 30 cases of normal endometrium of mediastinum were detected by immunohistochemistry and reverse transcription-PCR reverse polymerase chain reaction (RT-PCR).To compare the difference of OPN expression between uterine cavity adhesion group and normal group, and the difference of endometrial OPN expression in different degree of uterine cavity adhesion group, and to analyze the results.The result is 1: 1.The results of immunohistochemistry showed that the expression of OPN protein was mainly in endometrial glands. The expression of P0.05 IUA protein in interstitial tissue was higher than that in non-IUA group. The difference was statistically significant in severe IUA group than in moderate IUA group, and the expression of P0.05% OPN protein in severe IUA group was significantly higher than that in moderate IUA group.The expression of IUA in moderate IUA group was higher than that in mild IUA group, and the difference was statistically significant (P 0.05). 2.The results of reverse transcription-polymerase chain reaction showed that the expression of IUA was higher in IUA group than in non-#en1# group, the difference was statistically significant (P 0.05), the expression of IUA in severe IUA group was higher than that in moderate IUA group, and the expression in moderate IUA group was higher than that in mild IUA group.The difference was statistically significant (P 0.05), which was consistent with the results of immunohistochemistry.Conclusion: 1. The expression of OPN in endometrium of patients with intrauterine adhesion is significantly increased, suggesting that OPN may be related to the occurrence of intrauterine adhesions.With the severity of intrauterine adhesion, the expression of OPN was gradually increased, suggesting that OPN might be related to the severity of intrauterine adhesion.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R711.74

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