某地区生殖器人乳头状瘤病毒感染现状调查及基因亚型分析

发布时间：2018-04-09 03:38

本文选题：人乳头瘤病毒　切入点：基因型　出处：《现代预防医学》2015年02期

【摘要】：目的调查小榄地区生殖器人乳头状瘤病毒(HPV)感染情况及其基因亚型分析,为HPV感染分子流行病学研究和特异性疫苗的研制提供依据。方法采用凯普导流杂交HPV DNA检测法,对小榄地区疑似HPV感染的2 160例生殖器分泌物进行21种HPV亚型检测。结果 382例男性标本中有142例HPV检测阳性,感染率为37.17%。其中高危型占33.59%,低危型占57.25%,1 778例女性标本中有449例HPV检测阳性,感染率为25.25%,其中高危型占81.51%。女性HPV高危型感染率随着年龄的增长而升高(小于21岁除外)。男性HPV感染的高峰年龄为21~40岁,占90.14%,在31~40岁年龄段,HPV高危型感染率和低危型感染率差异无统计学意义(P0.05)。男性和女性的生殖器HPV高危亚型检出频率较高的前五位基因型别相同,除HPVl6型之外,其他4个型别52、58、31和33占高危型的68.04%(281/413)。结论小榄地区生殖器人乳头状瘤病毒感染较严重,男性和女性HPV感染的主要高危亚型都是16,52、58、31和33,这对小榄地区HPV分子流行病学的研究和相关肿瘤的防治具有重要意义。
[Abstract]:Objective to investigate the human papillomavirus (HPV) infection and its gene subtypes in Xiaolan area, and to provide a basis for the molecular epidemiology of HPV infection and the development of specific vaccine.Methods 21 HPV subtypes of 2 160 genital secretions from suspected HPV infection in Xiaolan area were detected by HPV DNA.Results 142 of 382 male specimens were positive for HPV, and the infection rate was 37.17%.Among them, high risk type accounted for 33.59m and low risk type accounted for 57.251.778 female specimens, 449 cases were positive for HPV, the infection rate was 25.25%, among which high risk type accounted for 81.51%.The infection rate of high risk type of HPV in women increased with age (except 21 years old).The peak age of male HPV infection was 21 ~ 40 years old (90.14). There was no significant difference between high risk and low risk HPV infection rate in the age group of 31 ~ 40 years old.Male and female genital HPV high risk subtypes were the same in the first five genotypes, except for HPVl6 type, the other four genitals were 68.04% 281R / 413G, except for HPVl6 type 58831 and 33.Conclusion the infection of human papillomavirus in genitalia in Xiaolan area is serious. The main high risk subtypes of HPV infection in male and female are 1652A5831 and 333.This is of great significance to the study of molecular epidemiology of HPV and the prevention and treatment of related tumors in Xiaolan area.
【作者单位】：南方医科大学附属小榄医院检验科;
【基金】：中山市医学科研基金项目(2013A020207)
【分类号】：R737.33

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