miR-23及孕激素受体蛋白在子宫肌瘤组织中表达及临床意义研究

发布时间：2018-04-10 00:07

本文选题：子宫肌瘤　切入点：miR-　出处：《中国实用妇科与产科杂志》2017年06期

【摘要】：目的探讨miR-23和孕激素受体(PR)蛋白在子宫肌瘤组织中的表达及临床意义。方法分别用realtimePCR法及western blot法对2013年12月至2014年6月中国医科大学附属盛京医院30例子宫肌瘤组织、30例相应肌瘤包膜外子宫肌层组织及10例因子宫脱垂行子宫切除术的子宫肌层组织中的miR-23和PR蛋白进行检测,并用SPSS17.0统计分析软件对检测结果进行统计及相关性分析。结果 miR-23在相应包膜外子宫肌层组及正常子宫肌层组表达显著高于子宫肌瘤组织(P0.001)。孕激素受体A(PRA)、孕激素受体B(PRB)蛋白在相应包膜外子宫肌层组及正常子宫肌层组表达显著低于子宫肌瘤组织(P0.001)。比较相应包膜外子宫肌层组与正常子宫肌层组中PRA、PRB蛋白的相对含量,PRA相对含量差异无统计学意义(P=0.198),PRB相对含量差异有统计学意义(P=0.035)。在子宫肌瘤组织中,PRA、PRB蛋白表达差异无统计学意义(P=0.389)。miR-23与PRA蛋白表达之间存在负相关关系(r=-0.754),与PRB蛋白相对含量之间无相关关系(r=-0.394)。结论 miR-23与PR共同促进子宫肌瘤的发生与发展。PR为miR-23的靶基因之一,miR-23可能通过转录水平导致PRA蛋白增多。
[Abstract]:Objective to investigate the expression and clinical significance of miR-23 and progesterone receptor protein in uterine leiomyoma.Methods from December 2013 to June 2014, 30 cases of uterine leiomyoma tissues and 10 cases of uterine myometrium tissues of Shengjing Hospital affiliated to China Medical University from December 2013 to June 2014 were treated with realtimePCR method and western blot method respectively, and 10 cases were treated with hysterectomy due to uterine prolapse.The miR-23 and PR proteins in the myometrium of the uterus were detected.SPSS17.0 statistical analysis software was used to analyze the correlation and statistics of the test results.Results the expression of miR-23 in the corresponding extracapsular myometrium group and normal uterine myometrium group was significantly higher than that in uterine myoma tissue (P 0.001).The expression of progesterone receptor Agna and progesterone receptor Bu PRB protein in the corresponding extracapsular myometrium group and normal uterine myometrium group was significantly lower than that in uterine leiomyoma tissue (P 0.001).There was no significant difference in the relative content of PRA between the corresponding extrauterine myometrium group and the normal myometrium group.There was no significant difference in the expression of PRB protein in uterine leiomyoma. There was a negative correlation between the expression of PRA protein and the expression of PRB protein. There was no correlation between PRB protein and the relative content of PRB protein.Conclusion miR-23 and PR can promote the occurrence and development of uterine leiomyoma. PR is one of the target genes of miR-23, which may lead to the increase of PRA protein through transcription level.
【作者单位】：中国医科大学附属盛京医院妇产科;济南市妇幼保健院;
【分类号】：R737.33

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