不明原因早期复发性流产患者蜕膜自然杀伤细胞对滋养层细胞系侵袭功能的影响

发布时间：2018-04-10 17:25

本文选题：蜕膜自然杀伤细胞(dNK) + 复发性流产(RSA)　；参考：《中华生殖与避孕杂志》2017年05期

【摘要】：目的研究早孕期复发性流产(RSA)患者与正常早孕妇女的蜕膜自然杀伤细胞(d NK)对滋养层细胞系HTR-8/SVneo侵袭能力的影响。方法收集正常早孕人工流产妇女(正常早孕组,n=10)和不明原因RSA患者(RSA组,n=8)的早孕蜕膜组织,用密度梯度离心法分离出蜕膜淋巴细胞,免疫磁珠法进一步分选出CD56+CD16-NK细胞,即蜕膜NK细胞,将蜕膜NK细胞与HTR-8/SVneo共培养24 h后,通过MTT法和Transwell侵袭实验检测蜕膜NK细胞对滋养层细胞系HTR8/SVneo黏附及侵袭能力的影响,用Real-time PCR检测共培养后HTR-8/SVneo细胞的MMP-2和MMP-9 mRNA水平的表达情况。结果与正常早孕妇女相比,不明原因早孕期RSA患者的蜕膜NK细胞对滋养层细胞系HTR8/SVneo侵袭能力有减弱作用,并使HTR-8/SVneo表达促侵袭分子MMP-2和MMP-9表达降低。结论妊娠早期局部母-胎界面d NK细胞的功能异常可能是导致不明原因RSA的一个原因。
[Abstract]:Objective to study the effect of decidual natural killer (NK) on the invasion of trophoblastic cell line HTR-8/SVneo in patients with recurrent early pregnancy (RSA) and normal early pregnant women.Methods the decidual tissues of women with normal early pregnancy induced abortion (normal early pregnancy group) and unexplained RSA group were collected. Decidual lymphocytes were isolated by density gradient centrifugation, and CD56 CD16-NK cells were further isolated by immunomagnetic beads.Decidual NK cells were co-cultured with HTR-8/SVneo for 24 h. The effects of decidual NK cells on the adhesion and invasion of trophoblastic cell line HTR8/SVneo were detected by MTT and Transwell invasion assay.The expression of MMP-2 and MMP-9 mRNA in co-cultured HTR-8/SVneo cells was detected by Real-time PCR.Results compared with normal early pregnant women, decidua NK cells of unexplained early pregnancy RSA patients decreased the invasiveness of trophoblastic cell line HTR8/SVneo, and decreased the expression of MMP-2 and MMP-9 in HTR-8/SVneo.Conclusion the abnormal function of d NK cells at the maternal-fetal interface in early pregnancy may be one of the causes of unexplained RSA.
【作者单位】：第四军医大学唐都医院生殖医学中心;第四军医大学免疫学教研室;
【基金】：国家自然科学基金面上项目(81370710,81571531)~~
【分类号】：R714.21

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