人脐带间充质干细胞对异位子宫内膜细胞增殖及凋亡的影响

发布时间：2018-04-11 11:36

本文选题：脐带 + 间质干细胞　；参考：《中国组织工程研究》2015年41期

【摘要】：背景:间充质干细胞可以分泌多种细胞因子和生长因子,促进周围细胞的存活,发挥旁分泌作用。目的:探讨人脐带间充质干细胞对异位子宫内膜细胞增殖及凋亡的影响。方法:分离培养人脐带间充质干细胞和异位子宫内膜细胞,以异位子宫内膜细胞单独培养作为对照组,人脐带间充质干细胞与异位子宫内膜细胞共培养作为观察组。培养24,48,72 h时采用MTT法检测异位子宫内膜细胞增殖情况,流式细胞仪检测异位子宫内膜细胞凋亡情况,RT-PCR检测异位子宫内膜细胞PTEN基因表达情况。结果与结论:与对照组比较,培养24,48,72 h时观察组异位子宫内膜细胞增殖受到显著抑制,亚二倍体峰占细胞总数的比例显著升高(P0.05)。随着时间的推移,观察组细胞抑制率逐渐下降,各时间点比较差异有显著性意义(P均0.05)。与同期对照组比较,观察组细胞PTEN基因表达显著上调(P0.05)。培养48,72 h观察组细胞PTEN基因表达显著高于培养24 h(P0.05)。以上结果表明人脐带间充质干细胞可以通过上调PTEN基因表达,抑制异位子宫内膜细胞增殖,并促进其凋亡。
[Abstract]:Background: mesenchymal stem cells can secrete many cytokines and growth factors to promote the survival of peripheral cells and play a paracrine role.Aim: to investigate the effects of human umbilical cord mesenchymal stem cells (HMSCs) on the proliferation and apoptosis of ectopic endometrial cells.Methods: human umbilical cord mesenchymal stem cells and ectopic endometrial cells were isolated and cultured. Ectopic endometrial cells were isolated as control group and human umbilical cord mesenchymal stem cells co-cultured with ectopic endometrial cells as observation group.The proliferation of ectopic endometrial cells was detected by MTT assay, apoptosis of ectopic endometrial cells was detected by flow cytometry and PTEN gene expression in ectopic endometrial cells was detected by reverse transcription-polymerase chain reaction (RT-PCR).Results and conclusion: compared with the control group, the proliferation of ectopic endometrial cells was significantly inhibited and the ratio of the hypodiploid peak to the total number of cells was significantly increased in the observation group at 24 h for 48 h 72 h.With the passage of time, the cell inhibition rate in the observation group decreased gradually, and there were significant differences between the different time points (P < 0.05).Compared with the control group, the expression of PTEN gene increased significantly in the observation group.The expression of PTEN gene in observation group was significantly higher than that in 24 h culture group (P 0.05).These results suggest that human umbilical cord mesenchymal stem cells can inhibit the proliferation and promote the apoptosis of ectopic endometrial cells by upregulating the expression of PTEN gene.
【作者单位】：青海红十字医院;
【分类号】：R711.71

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