HPV16型E6蛋白的B细胞线性表位预测及分析

发布时间：2018-04-11 13:11

本文选题：HPV型 + E蛋白　；参考：《中国病原生物学杂志》2015年03期

【摘要】：目的对高危型人乳头瘤病毒(Human papillomavirus)16型E6蛋白的B细胞线性表位进行预测。方法以HPV16型E6蛋白(P03126)的全长氨基酸序列为基础,采用EXPASY网站上的生物信息学软件和DNAstar软件包中的Protean软件分析E6蛋白的二级结构,跨膜趋势及其亲水性,表面可及性,抗原性,极性和柔韧性等特性。综合以上参数预测HPV16型E6蛋白的B细胞线性表位,并进一步进行同源性匹配分析。结果综合多个参数分析显示B细胞线性表位可能位于E6蛋白N端2-7、9-19、30-36、45-49、105-109、119-125和148-158区段及其附近,进一步同源性匹配分析支持其中区段2-7、9-19、119-125及148-158为可能的B细胞线性表位,其中区段148-158(RSSRTRRETQL)为HPV16型E6蛋白所特有的序列,而区段2-7(HQKRTA)、9-19(FQDPQERPRKL)与HPV9型E6蛋白具有同源性,区段119-125(PEEKQRH)与HPV31型E6蛋白具有同源性。结论综合分析预测HPV16型E6蛋白的B细胞线性表位可能位于E6蛋白N端2-7、9-19、119-125及148-158区段,为HPV16型E6蛋白的进一步研究奠定了基础。
[Abstract]:Objective to predict B cell linear epitopes of high risk human papillomavirus human papillomavirus)16 E6 protein.Methods based on the full-length amino acid sequence of HPV16 E6 protein P03126, the secondary structure, transmembrane trend, hydrophilicity, surface accessibility and antigenicity of E6 protein were analyzed by using bioinformatics software on EXPASY website and Protean software in DNAstar software package.Polarity and flexibility.The B cell linear epitopes of HPV16 type E6 protein were predicted by the above parameters, and the homology matching analysis was carried out.Results multiple parameter analysis showed that the B cell linear epitopes might be located at and around the N-terminal of E6 protein, and further homology matching analysis suggested that the B cell linear epitopes of regions 2-7n9-19119-125 and 148-158 might be located in and around the N-terminal of E6 protein, and that the B cell linear epitopes might be located in the N-terminal of E6 protein, and further homology matching analysis showed that the B cell linear epitopes were located in regions 2-79-19119-125 and 148-158, respectively.Sequence 148-158RSSRTRRETQL) is a unique sequence of HPV16 type E6 protein, while region 2-7 HQKRTAKL (9-19 FQDQERPRKL) is homologous to HPV9 type E6 protein, and region 119-125 (PEEKQRH) is homologous to HPV31 type E6 protein.Conclusion the B cell linear epitopes of HPV16 type E6 protein may be located at the N-terminal of E6 protein 2-79-19119-125 and 148-158, which lays a foundation for the further study of HPV16 type E6 protein.
【作者单位】：温州医科大学第一临床医学院;温州医科大学分子病毒与免疫研究所微生物学与免疫学教研室;
【基金】：国家自然科学基金项目(No.81172463)
【分类号】：R737.33

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