子宫内膜腺癌组织中IGFBP-rP1基因的甲基化状态及其mR-NA、蛋白表达水平的检测

发布时间：2018-04-12 10:10

本文选题：子宫内膜腺癌 + 胰岛素样生长因子结合蛋白相关蛋白　；参考：《郑州大学学报(医学版)》2015年06期

【摘要】：目的:探讨子宫内膜腺癌组织中胰岛素样生长因子结合蛋白相关蛋白1(IGFBP-r P1)基因的甲基化状态及其与IGFBP-r P1 mRNA、蛋白表达之间的关系。方法:采用甲基化特异性PCR检测45例子宫内膜腺癌、30例子宫内膜单纯性增生和30例子宫内膜不典型增生组织中IGFBP-r P1基因启动子区和第一外显子区Cp G岛的甲基化状态;采用实时荧光定量PCR和免疫组化SP法分别检测上述3种组织中IGFBP-r P1 mRNA和蛋白的表达。结果:子宫内膜腺癌组织中IGFBP-r P1基因在启动子区的甲基化率高于其在第一外显子区的甲基化率(χ2=6.429,P=0.011)。子宫内膜腺癌组织中IGFBP-r P1基因在启动子区的甲基化率低于子宫内膜不典型增生组织和单纯性增生组织(F=14.659,P=0.001)。子宫内膜腺癌组织中IGFBP-r P1 mRNA和蛋白的表达均高于子宫内膜不典型增生组织和单纯性增生组织(F=8.619、χ2=23.611,P均0.05);在启动子区发生IGFBP-r P1基因甲基化的子宫内膜腺癌组织中,其mRNA的表达水平低于未甲基化组(t=4.758,P=0.001),其蛋白的表达与甲基化状态呈负关联(rP=-0.625,P0.001)。结论:子宫内膜腺癌组织中IGFBP-r P1基因甲基化主要发生在启动子区,且呈低甲基化状态;IGFBP-r P1启动子区的低甲基化状态可能是IGFBP-r P1基因在子宫内膜腺癌组织中高表达的调控机制之一。
[Abstract]:Aim: to investigate the methylation status of insulin-like growth factor binding protein-associated protein (1(IGFBP-r P1) gene in endometrial adenocarcinoma and its relationship with the expression of IGFBP-r P1 mRNAs and proteins.Methods: methylation-specific PCR was used to detect the methylation status of CP G islands in the promoter region and the first exon region of IGFBP-r P1 gene in 30 cases of endometrial simple hyperplasia and 30 cases of endometrial atypical hyperplasia in 45 cases of endometrial adenocarcinoma and 30 cases of endometrial atypical hyperplasia.The expression of IGFBP-r P1 mRNA and protein in these three tissues was detected by real-time fluorescence quantitative PCR and immunohistochemical SP method.Results: the methylation rate of IGFBP-r P1 gene in the promoter region of endometrial adenocarcinoma was higher than that in the first exon (蠂 2 6.429).The methylation rate of IGFBP-r P1 gene in endometrial adenocarcinoma was lower than that in atypical endometrial hyperplasia and simple hyperplasia.The expression of IGFBP-r P1 mRNA and protein in endometrial adenocarcinoma was higher than that in atypical endometrial hyperplasia and simple hyperplasia (P < 0.05).The expression level of mRNA was lower than that of unmethylated group, and the protein expression was negatively correlated with methylation status.Conclusion: the methylation of IGFBP-r P1 gene mainly occurs in the promoter region of endometrial adenocarcinoma.The hypomethylation of IGFBP-r P1 promoter may be one of the regulatory mechanisms of IGFBP-r P1 gene overexpression in endometrial adenocarcinoma.
【作者单位】：河南省高等学校临床医学重点学科开放实验室;
【基金】：河南省卫生科技创新型人才工程基金资助项目豫卫科[2010]52号
【分类号】：R737.33

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