外加磁场对SPIO-shRNA分子探针转染卵巢癌SKOV3细胞的影响

发布时间：2018-04-14 00:00

本文选题：分子探针 + 磁场　；参考：《中国医学科学院学报》2015年01期

【摘要】：目的探讨外加磁场对SPIO-shRNA分子探针转染人卵巢癌SKOV3细胞的影响。方法设定质量浓度为45 mg/L的SPIO-shRNA分子探针转染人卵巢癌SKOV3细胞,并将SKOV3细胞分为3组:未转染的细胞组(空白对照组),SPIO-shRNA分子探针转染的细胞组(阴性对照组),培养皿底部外加磁场的SPIO-shRNA分子探针转染的细胞组(实验组)。采用流式细胞术检测细胞转染率,CCK-8法检测细胞增殖及活性,Western blot法检测细胞表皮生长因子受体(EGFR)蛋白水平,荧光定量PCR检测EGFR mRNA水平,磁共振(MR)扫描检测细胞内信号强度变化。结果与阴性对照组比较,实验组的细胞转染率[(79.20±3.31)%]显著增加(P=0.001);实验组的细胞活性(P=0.011)、EGFR蛋白及mRNA表达量均明显降低(P均0.05);实验组的MR图像信号强度明显降低(P=0.0004)。结论外加磁场可以诱导SPIO-shRNA分子探针进入卵巢癌SKOV3细胞,从而提高细胞的转染率。
[Abstract]:Objective to investigate the effect of magnetic field on SPIO-shRNA molecular probe was transfected into human ovarian cancer cell line SKOV3. The concentration setting method for 45 mg/L SPIO-shRNA molecular probe was transfected into human ovarian cancer SKOV3 cells, and SKOV3 cells were divided into 3 groups: untransfected cells group (control group), group of SPIO-shRNA cells transfected with negative (molecular probes the control group), group SPIO-shRNA cell culture molecular probe transfected dish bottom of applied field (experimental group). The transfection rate were detected by flow cytometry, cell proliferation and CCK-8 activity detection method, the epidermal growth factor receptor Western detection method of blot (EGFR) protein level, EGFR fluorescence quantitative PCR to detect the level of mRNA, magnetic resonance (MR) signal intensity changes of scan cells. Results compared with the control group, transfection rate of the experimental group [(79.20 + 3.31) when it was significantly increased (P=0.001); cell activity of experimental group (P=0.0 11), the expression of EGFR protein and mRNA decreased significantly (P all 0.05). The MR image signal intensity of the experimental group was significantly decreased (P=0.0004). Conclusion the external magnetic field can induce SPIO-shRNA probe to enter the SKOV3 cell of ovarian cancer, thereby enhancing the transfection rate of cells.

【作者单位】：重庆医科大学附属第一医院放射科;
【基金】：国家自然科学基金(81171366) 国家临床重点专科建设项目(2013-544)~~
【分类号】：R737.31

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