鼠胚实验在新建体外受精胚胎培养室质量控制中的作用

发布时间：2018-04-14 04:26

本文选题：鼠胚实验 + 体外受精　；参考：《实用妇产科杂志》2015年02期

【摘要】：目的:检测新建体外受精实验室培养体系的可靠与否,是否符合人类胚胎体外培养的要求。方法:对昆明白小鼠进行超促排卵,手术获取昆明白小鼠雌雄配子进行体外受精和体内受精的胚胎;并进行体外培养观察两组鼠胚的受精率、2-细胞率、囊胚率及采用密度梯度离心法和上游法两种精液处理方法对小鼠体外受精胚胎发育情况的影响。结果:进行了30个周期的体内受精体外培养实验,共获卵1136枚;进行了35个周期的体外受精实验,共获卵1486枚。体内受精组获得的受精率(93.13±1.46)%和2-细胞率(94.52±1.67)%高于体外受精组的受精率(84.39±2.87)%和2-细胞率(87.80±3.21)%,两组间差异有统计学意义(P0.05)。密度梯度离心法处理精液后,其受精率(90.32±3.14)%和2-细胞率(88.54±2.28)%高于上游法的受精率(76.46±2.35)%和2-细胞率(82.67±2.62)%,两者差异有统计学意义(P0.05)。结论:小鼠卵母细胞体内受精和体外受精后均有高的成胚率,体内受精后进行体外培养优于体外受精后培养,采用密度梯度离心处理精液实施体外受精优于上游法,鼠胚实验可对新建胚胎培养室的培养体系进行评估。
[Abstract]:Aim: to determine whether the new culture system of in vitro fertilization laboratory meets the requirements of in vitro culture of human embryos.Methods: the embryos of male and female gametes of Kunming white mice for in vitro fertilization and in vivo fertilization were obtained by hyperstimulation of ovulation in Kunming white mice, and the fertilization rate and 2 cell rate of mouse embryos in both groups were observed by in vitro culture.Effect of blastocyst rate and semen treatment by density gradient centrifugation and upstream method on in vitro fertilization and embryo development in mice.Results: a total of 1136 eggs and 1486 eggs were obtained from 30 cycles of in vitro fertilization and 35 cycles of in vitro fertilization.The fertilization rates of 93.13 卤1.46% and 94.52 卤1.67% in IVF group were significantly higher than those in IVF group (84.39 卤2.87% and 87.80 卤3.21%). The difference between the two groups was statistically significant (P 0.05).The fertilization rates of semen treated by density gradient centrifugation were 90.32 卤3.14% and 88.54 卤2.28%, respectively, which were significantly higher than those of upstream method (76.46 卤2.35% and 82.67 卤2.62%).Conclusion: the in vivo fertilization and in vitro fertilization of mouse oocytes have a high rate of embryo formation, and the in vitro culture after in vivo fertilization is better than that after in vitro fertilization, and the in vitro fertilization with density gradient centrifugation is superior to the upstream method.The mouse embryo experiment can evaluate the culture system of the new embryo culture room.
【作者单位】：中山大学附属第三医院生殖医学中心;
【分类号】：R714.8

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