顺铂与紫杉醇联合应用对小鼠宫颈癌组织中Bcl-2、Bax、Caspase-9表达的影响

发布时间：2018-04-17 05:41

  本文选题：顺铂 + 紫杉醇　； 参考：《山东医药》2015年23期

【摘要】：目的观察顺铂(DDP)与紫杉醇(TXL)联合应用对宫颈癌小鼠宫颈癌组织Bcl-2、Bax、半胱氨酸蛋白酶9(Caspase-9)表达的影响。方法 32只615小鼠均建立小鼠宫颈癌模型,后随机分为A组9只、B组9只、C组10只、D组10只。A组小鼠皮下注射生理盐水0.2 m L,每6天1次。B组小鼠皮下注射DDP 4 mg/(kg·m L),每6天1次。C组小鼠腹腔注射TXL 50 mg/(kg·m L),每周1次。D组小鼠皮下注射DDP 4 mg/(kg·m L),每6天1次;TXL 50 mg/(kg·m L),每周1次;第1次注射时先注射TXL后注射DDP,其后两药序贯用药。采用免疫组化SP法检测各组Bcl-2、Bax、Caspase-9。结果 A组宫颈癌组织Bcl-2表达量为6 300.43±458.34、Bax表达量为7 244.22±363.72、Bax/Bcl-2为1.14±0.31、Caspase-9表达量为5 144.22±363.71,B组分别为4 146.56±202.50、6 613.89±237.49、1.59±0.22、6 913.89±337.49,C组分别为5 361.20±367.80、6 210.50±320.25、1.15±0.31、9 310.50±220.25,D组分别为1 950.80±103.31、9 334.40±471.53、4.79±1.34、10 334.40±351.53,A、B、C组分别与D组比较,P均0.05。结论与单用DDP、TXL比较,DDP联合TXL化疗可明显降低Bcl-2水平,增加Bax蛋白水平和激活Caspase-9,从而达到促进宫颈癌细胞凋亡的作用。
[Abstract]:Objective to observe the effect of cisplatin (DDP) combined with paclitaxel (TXL) on the expression of Bcl-2 BaxCaspase-9 in cervical carcinoma of mice.Methods the cervical cancer models were established in 32 615 mice.Then it was randomly divided into group A (n = 9), group C (n = 9), group D (n = 10), group D (n = 10), group D (n = 10), group A (n = 10), group A (n = 10), group A (n = 10), group A (n = 10), group A (n = 10), group A (n = 10), group A (n = 10), group A (n = 10), group A (n =Mice in group D were subcutaneously injected with DDP 4 mg/(kg mL once a week and treated with TXL 50 mg/(kg mL once every 6 days once a week.At the first injection, TXL was injected first, and then the two drugs were used sequentially.Immunohistochemical SP method was used to detect Caspase-9 of Bcl-2 and Baxia in each group.缁撴灉 A缁勫��棰堢檶缁勭粐Bcl-2琛ㄨ揪閲忎负6 300.43卤458.34,Bax琛ㄨ揪閲忎负7 244.22卤363.72,Bax/Bcl-2涓，

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